Plant Soil Environ., 2012, 58(10):441-445 | DOI: 10.17221/305/2012-PSE

Effects of homobrassinolide in barley callus culture

A. Temel, N. Gozukirmizi
Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Vezneciler, Istanbul, Turkey

Thirty-day-old barley (Hordeum vulgare L. cv. Zafer-160) calli were treated with 0, 0.5, 1 µmol/L 22(S),23(S)-Homobrassinolide (HBL) for four weeks. Transfer of calli to hormone-free or HBL-supplemented medium caused an increase in total soluble protein and DNA contents and a decrease in superoxide dismutase (SOD; E.C. 1.15.1.1) activity. 0.5 µmol/L HBL caused the highest protein and DNA levels and the minimum decrease in fresh weight; while the lowest superoxide dismutase activity was recorded in 1 µmol/L HBL-treated group. Calli developed nodular structures and green cell clusters after transfer onto hormone-free or HBL-supplemented medium. Methylation-sensitive restriction fingerprinting (MSRF) analysis detected a few changes in methylation between control and 0.5 µmol/L groups. Combination of data led us to the conclusion that HBL favoured regeneration and shoot development and may ameliorate tissue culture stress in a mechanism, which is independent of cytosine methylation.

Keywords: brassinosteroids; superoxide dismutase; regeneration; MSRF; methylation

Published: October 31, 2012  Show citation

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Temel A, Gozukirmizi N. Effects of homobrassinolide in barley callus culture. Plant Soil Environ. 2012;58(10):441-445. doi: 10.17221/305/2012-PSE.
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