Plant Soil Environ., 2016, 62(2):92-97 | DOI: 10.17221/770/2015-PSE

Dynamics of microbial population size in rhizosphere soil of Monsanto's Cry1Ac cottonOriginal Paper

Y.-J. Zhang, M. Xie, D.-L. Peng, J.-J. Zhao, Z.-R. Zhang
State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory for Biological Control of Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China

Monsanto's Bt-cotton NC 33B, planted in northern China for more than one decade, effectively controls cotton bollworms; however the understanding of its potential effects on soil microorganisms is limited. The dynamics of eubacterial, fungal and actinomycetes population sizes in rhizosphere soil of the Bt cotton were analysed by real-time PCR (qPCR) at the different growth stages under field conditions during 2009 to 2011. Results showed that the population sizes (microbial rDNA gene copies) of eubacteria, fungi and actinomycetes in rhizosphere soil were markedly affected by natural variations in the environment related to the year, cotton growth and cultivar. However, there was no significant difference in eubacterial, fungal and actinomycetes population size in rhizosphere soil between the Bt-cotton NC 33B and its near-isogenic comparator DP 5415. In general, the Bt-cotton NC 33B did not show evident effects on the population sizes of eubacteria, fungi and actinomycetes in rhizosphere soil under field conditions after three-year cultivation.

Keywords: Bt protein; soil ecosystem; microbial community; Gossypium hirsutum L.

Published: February 29, 2016  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Zhang Y-J, Xie M, Peng D-L, Zhao J-J, Zhang Z-R. Dynamics of microbial population size in rhizosphere soil of Monsanto's Cry1Ac cotton. Plant Soil Environ. 2016;62(2):92-97. doi: 10.17221/770/2015-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Barriuso J., Valverde J.R., Mellado R.P. (2012): Effect of Cry1Ab protein on rhizobacterial communities of Bt-maize over a fouryear cultivation period. PloSONE, 7: e35481. Go to original source... Go to PubMed...
    2. Castaldini M., Turrini A., Sbrana C., Benedetti A., Marchionni M., Mocali S., Fabiani A., Landi S., Santomassimo F., Pietrangeli B., Nuti M.P., Miclaus N., Giovannetti M. (2005): Impact of Bt corn on rhizospheric and soil eubacterial communities and on beneficial mycorrhizal symbiosis in experimental microcosms. Applied and Environmental Microbiology, 71: 6719-6729. Go to original source... Go to PubMed...
    3. Donegan K.K., Palm C.J., Fieland V.J., Porteous L.A., Ganio L.M., Schaller D.L., Bucao L.Q., Seidler R.J. (1995): Changes in levels, species and DNA fingerprints of soil microorganisms associated with cotton expressing the Bacillus thuringiensis var. kurstaki endotoxin. Applied Soil Ecology, 2: 111-124. Go to original source...
    4. Donegan K.K., Shaller D.L., Stone J.K., Ganio L.M., Reed G., Hamm P.B., Seidler R.J. (1996): Microbial populations, fungal species diversity and plant pathogen levels in field plots of potato plants expressing the Bacillus thuringiensis var. tenebrionis endotoxin. Transgenic Research, 5: 25-35. Go to original source...
    5. Fierer N., Jackson J.A., Vilgalys R., Jackson R.B. (2005): Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays. Applied and Environmental Microbiology, 71: 4117-4120. Go to original source... Go to PubMed...
    6. Hannula S.E., de Boer W., van Veen J. (2012): A 3-year study reveals that plant growth stage, season and field site affect soil fungal communities while cultivar and GM-trait have minor effects. PloSONE, 7: e33819. Go to original source... Go to PubMed...
    7. Icoz I., Saxena D., Andow D.A., Zwahlen C., Stotzky G. (2008): Microbial populations and enzyme activities in soil in situ under transgenic corn expressing Cry proteins from Bacillus thuringiensis. Journal of Environmental Quality, 37: 647-662. Go to original source... Go to PubMed...
    8. James C. (2014): Global Status of Commercialized Biotech/GM crops: 2014. ISAAA Brief No. 49. Ithaca, International Service for the Acquisition of Agri-biotech Applications.
    9. Li X.G., Liu B., Cui J.J., Liu D.D., Ding S., Gilna B., Luo J.Y., Fang Z.X., Cao W., Han Z.M. (2011): No evidence of persistent effects of continuously planted transgenic insect-resistant cotton on soil microorganisms. Plant and Soil, 339: 247-257. Go to original source...
    10. Munger P., Bleiholder H., Hack H., Hess M., Stauss R., van den Boom T., Weber E. (1998): Phenological growth stages of the cotton plant (Gossypium hirsutum L.): Codification and description according to the BBCH scale. Journal of Agronomy and Crop Science, 180: 143-149. Go to original source...
    11. Na R.S., Yu H., Yang D.L., Zhao J.N., Li G., Na B.Q., Liu L. (2011): Effect of plantation of transgenic Bt cotton on the amount of rhizospheric soil microorganism and bacterial diversity in the cotton region of Yellow River basin. Chinese Journal of Applied Ecology, 22: 114-120. Go to PubMed...
    12. Pindi P.K., Sultana T. (2013): Bacterial and fungal diversity in rhizosphere soils of Bt and non-Bt cotton in natural systems. Bulgarian Journal of Agricultural Science, 19: 1306-1310.
    13. Saxena D., Flores S., Stotzky G. (2002): Bt toxin is released in root exudates from 12 transgenic corn hybrids representing three transformation events. Soil Biology and Biochemistry, 34: 133-137. Go to original source...
    14. Saxena D., Stotzky G. (2000): Insecticidal toxin from Bacillus thuringiensis is released from roots of transgenic Bt corn in vitro and in situ. FEMS Microbiology Ecology, 33: 35-39. Go to original source... Go to PubMed...
    15. Shen R.F., Cai H., Gong W.H. (2006): Transgenic Bt cotton has no apparent effect on enzymatic activities or functional diversity of microbial communities in rhizosphere soil. Plant and Soil, 285: 149-159. Go to original source...
    16. Singh A.K., Singh M., Dubey S.K. (2013): Changes in actinomycetes community structure under the influence of Bt transgenic brinjal crop in a tropical agroecosystem. BMC Microbiology, 13: 122. Go to original source... Go to PubMed...
    17. Singh A.K., Singh M., Dubey S.K. (2014): Rhizospheric fungal community structure of a Bt brinjal and a near isogenic variety. Journal of Applied Microbiology, 117: 750-765. Go to original source... Go to PubMed...
    18. Stotzky G. (2004): Persistence and biological activity in soil of the insecticidal proteins from Bacillus thuringiensis, especially from transgenic plants. Plant and Soil, 266: 77-89. Go to original source...
    19. Visser S., Parkinson D. (1992): Soil biological criteria as indicators of soil quality: Soil microorganisms. American Journal of Alternative Agriculture, 7: 33-37. Go to original source...
    20. Wei M., Tan F., Zhu H., Cheng K., Wu X., Wang J., Zhao K., Tang X. (2012): Impact of Bt-transgenic rice (SHK601) on soil ecosystems in the rhizosphere during crop development. Plant, Soil and Environment, 58: 217-223. Go to original source...
    21. Wu K.M., Guo Y.Y., Lv N., Greenplate J.T., Deaton R. (2003): Efficacy of transgenic cotton containing a cry1Ac gene from Bacillus thuringiensis against Helicoverpa armigera (Lepidoptera: Noctuidae) in northern China. Journal of Economic Entomology, 96: 1322-1328. Go to original source... Go to PubMed...
    22. Wu W.X., Liu W., Lu H.H., Chen Y.X., Medha D., Janice T. (2009): Use of 13C labeling to assess carbon partitioning in transgenic and nontransgenic (parental) rice and their rhizosphere soil microbial communities. FEMS Microbiology Ecology, 67: 93-102. Go to original source... Go to PubMed...
    23. Xiao Y., Zheng G.M., Yang Z.H., Ma Y.H., Huang C., Xu Z.Y., Huang J., Fan C.Z. (2010): Changes in the actinomycetal communities during continuous thermophilic composting as revealed by denaturing gradient gel electrophoresis and quantitative PCR. Bioresource Technology, 102: 1383-1388. Go to original source... Go to PubMed...
    24. Zhang Y.J., Xie M., Peng D.L. (2013): Effects of transgenic crops on soil microorganisms: A review. Chinese Journal of Applied Ecology, 24: 2685-2690. Go to PubMed...
    25. Zhang Y.J., Xie M., Wu G., Peng D.L., Yu W.B. (2015): A 3-year field investigation of impacts of Monsanto's transgenic Bt-cotton NC 33B on rhizosphere microbial communities in northern China. Applied Soil Ecology, 89: 18-24. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content