Plant, Soil and Environment, 2002 (vol. 48), issue 11
Analysis of chromosome termini in potato varieties
J. Fajkus, M. Novotná, J. Ptáček
Plant Soil Environ., 2002, 48(11):477-479 | DOI: 10.17221/4398-PSE 
Chromosomes of potato (Solanum tuberosum) are terminated by telomeres, which are formed by tandemly repeated [TTTAGGG]n oligonucleotide sequence. The total length of blocks of telomeric DNA has been known to vary largely among plant species and their varieties, and also among individual chromosome arms within a single nucleus. To check for such differences in potato varieties, which could be of a possible use in genotyping, we performed pulsed-field gel electrophoretic analysis of terminal restriction fragments in selected potato varieties. We found a typical range of telomere lengths of 20-60 kb in most analysed varieties. In one of analysed...
Segregation of DNA markers of potato (Solanum tuberosum ssp. tuberosum L.) resistance against Ro1 pathotype Globodera rostochiensis in selected F1 progeny
S. Skupinová, P. Vejl, P. Sedlák, J. Domkářová
Plant Soil Environ., 2002, 48(11):480-485 | DOI: 10.17221/4399-PSE
Marker assisted selection is the fast and objective method for detection of resistance major genes. This method is practical for identification of some candidate genes of quantitative resistance. Genetic markers based on Polymerase Chain Reaction (PCR) were used for evaluation of F1 progenies Ornella × Mira and Tábor × Mira. Cultivar Mira has resistance against Ro1 pathotype G. rostochiensis. Cultivars Ornela and Tábor are susceptible to Ro1. Seedlings of F1 generations were used for analyses. Plants were cultivated in greenhouse. DNA was isolated from tissue discs by GenElute Plant Genomic DNA Kit (Sigma, SRN). PCR marker...
Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism
P. Vejl, S. Skupinová, P. Sedlák, J. Domkářová
Plant Soil Environ., 2002, 48(11):486-489 | DOI: 10.17221/4400-PSE
Progressive methods of molecular analyses of DNA are routinely used in the fields of zoological and botanical taxonomy, pest management and plant breeding. Knowledge of species-composition in populations of potato cyst nematodes (Globodera rostochiensis, G. pallida) is very important for selection of appropriate measure of regulation PCN's occurrence. The molecular method for distinguishing of PCN species is described in this article. European populations of PCN - Šluknov (Ro1), Obersteinbach (Ro2), Harmerz (Ro5), Kalle (Pa2), Chavornay (Pa3), Delmsen (Pa3), and some cysts of unknown pathotype from Kašperské hory (K) locality were...
Abscisic acid content during cold hardening of barley and wheat cultivars with different freezing toler
Z. Faltusová-Kadlecová, M. Faltus, I. Prášil
Plant Soil Environ., 2002, 48(11):490-493 | DOI: 10.17221/4401-PSE
Endogenous content of abscisic acid was studied in a set of two winter cultivars of barleys (Lunet, Cenader), one spring cultivar (Akcent) and five winter cultivars of wheat (Mironovská, Samanta, Šárka, Zdar, Apache) and one spring cultivar (Leguan) in the course of cold hardening of hydroponically grown plants. Freezing tolerance was also determined in all barley and wheat cultivars under study. In none of the barley varieties did cold hardening of plants induce any significant change in abscisic acid content. In wheat plants exposed to cold hardening, the cultivars Apache and Leguan showed a slight transitory increase in abscisic acid content. Abscisic...
Influence of fertilization rates on species composition, quality and yields of the meadow fodder
J. Mrkvička, M. Veselá
Plant Soil Environ., 2002, 48(11):494-498 | DOI: 10.17221/4402-PSE
Four variants in four replications were studied on permanent meadow stand on Czech and Moravian highlands. The altitude a.s.l. is 485 m, original growth type was Dactylidetum, association Trifolio-Festucetum rubrae. Four variants: control (without fertilization), 40 kg P + 100 kg K/ha, 100 kg N/ha (+ PK) and 200 N/ha (+ PK) were evaluated. Botanical analyses of stands were done by the method of reduced projective dominance (D in %). The cut green matter was weighed up, mean samples for dry matter determination and chemical analyses were taken. Botanical composition of the meadow stand in 1991-2000 was not stabile and was changing...
Changes of botanical composition of grass stands under different types of management
J. Šantrůček, M. Svobodová, V. Brant
Plant Soil Environ., 2002, 48(11):499-504 | DOI: 10.17221/4403-PSE
A field trial with Bromus catharticus Vahl. cv. Tacit, Arrhenatherum elatius (L.) P. Beauv. ex J.S. et K.B. Presl cv. Median, Festuca pratensis Huds. cv. Otava and Dactylis aschersoniana Graebn. cv. Tosca sown in rows 125 mm, was established in the year 1996 in Prague (chernozem, altitude 281 m a.s.l., average precipitation 472 mm per year, average year temperature 9.3°C). The stands were cut one or three times per year. The mass was removed or once or twice mulched. The share of the botanical species (by weight method), number of present species and agrobotanical groups (grasses, legumes, other dicotyledonous) coverage...
Rapid hydroponic screening for molybdenum tolerance in rice through morphological and biochemical analysis
G.R. Rout, P. Das
Plant Soil Environ., 2002, 48(11):505-512 | DOI: 10.17221/4404-PSE
High yielding varieties of rice (Oryza sativa) cultivars were tested for their tolerance to different levels of molybdenum (Mo) (0.1µM - control, 0.2, 0.4, 0.8 and 1.6µM) in nutrient solution at pH 6.8. Seeds of rice were germinated and grown in presence of molybdenum under controlled environmental conditions. Standard growth parameters such as root length, shoot length, root/shoot dry biomass and root/shoot tolerance index were tested as markers of molybdenum toxicity. Measurements as early as 48 hours after the germination did not yield consistent results. However, root measurement on 3rd, 6th and 9th...
New hop variety Agnus as the result of breeding process innovation in the Czech Republic
V. Nesvadba, K. Krofta
Plant Soil Environ., 2002, 48(11):513-517 | DOI: 10.17221/4405-PSE
In the 90th, a methodology of hop breeding was innovated in the Czech Republic. Registration of a new variety Agnus in 2001 represents the result of the innovation mentioned above. Agnus variety is the first high-alpha hop in the assortment of Czech hop varieties. It contents 11-15% w/w of a-bitter acids and 5-7.5% w/w of b-bitter acids. The yield usually exceeds 2 t/ha. Cultivation of Agnus is tested in various localities within hop growing areas. Simultaneously brewing tests in pilot and full scale are performed in five Czech breweries.
The zinc mobility in three different soils amended by sewage sludge incubated with limestone and lime, and Zn uptake by oats
A. Hanč, P. Tlustoš, J. Száková, J. Balík, D. Pavlíková
Plant Soil Environ., 2002, 48(11):518-524 | DOI: 10.17221/4406-PSE
The effect of sewage sludge application on three soils of different properties (Chernozems - location Suchdol, Luvisols - location Červený Újezd and Cambisols - location Humpolec) was investigated in pot experiment and the accumulation of Zn in above ground biomass of oat as well as availability of Zn released by 0.01 mol/l CaCl2 was evaluated. Stabilised sewage sludge was obtained from three wastewater factories in Czech Republic. Sewage sludge has been preincubated with addition of limestone and lime (7% of Ca w/w of sludge dry solid) in aerobic and anaerobic conditions and tested in pot experiment. The highest Zn mobility reduction was...