Plant, Soil and Environment, 2005 (vol. 51), issue 2
Resistance to salinity stress and available water levels at the seedling stage of the common vetch (Vicia sativa L.)
A. Orak, E. Ateş
Plant Soil Environ., 2005, 51(2):51-56 | DOI: 10.17221/3555-PSE 
This research was carried out in the Field Crop Department, Tekirda Agriculture Faculty, and Plant Breeding Application and Research Centre, Trakya University, Turkey. The response of common vetch (Vicia sativa L.) seedlings to salt and available water level tolerance were compared for its water retention capability and the dry matter of shoots, root and shoot weights after 2 h incubation at 30°C and 1 h incubation at 105°C under the conditions of absence, as well as the presence of various levels of salinity and available water. Germination was not affected by the salinity and available water treatments. 1.35 dS/m salinity water treatment...
The antioxidative response of two tomato species with different drought tolerances as a result of drought and cadmium stress combinations
S. Ünyayar, Y. Keleş, F.Ö. Çekiç
Plant Soil Environ., 2005, 51(2):57-64 | DOI: 10.17221/3556-PSE
The effects of drought, cadmium (Cd) and drought-Cd combinations on antioxidant compounds, antioxidant enzymes and shoot growth were investigated for drought tolerant [Lycopersicon peruvianum (L.) Mill.] and sensitive of (Lycopersicon esculentum Mill. cv. Lukullus) tomato species. Drought-Cd combinations significantly decreased shoot growth in both species (P < 0.01), drought stress also decreased shoot growth in drought sensitive L. esculentum. Cd was accumulated higher in the roots of drought sensitive L. esculentum than drought tolerant L. peruvianum. The chlorophyll contents decreased...
Alleles controlling apple skin colour and incompatibility in new Czech apple varieties with different degrees of resistance against Venturia inaequalis CKE.
M. Melounová, P. Vejl, P. Sedlák, J. Blažek, J. Zoufalá, Z. Milec, H. Blažková
Plant Soil Environ., 2005, 51(2):65-73 | DOI: 10.17221/3557-PSE
The skin colour of 21 varieties was assessed on a molecular level. Four varieties had yellow skin colour with the genotype constitution a1a1, a1a2 or a2a2. Seventeen varieties had dominant red colour. Homozygous A1A1 constitution was present in 7 varieties, and heterozygous constitution A1a1 or A1a2 was found in the rest of the assessed varieties. The S-alleles controlled the incompatibility system of the pistil. Their detection is possible by means of the PCR method on the basis of allele specific primers. Six S-alleles...
The evaluation method of potato genotype resistance to blackspot bruise
J. Domkářová, B. Vokál
Plant Soil Environ., 2005, 51(2):74-81 | DOI: 10.17221/3558-PSE
In 2001-2003 a method for the evaluation of potato genotype (genetic resource) resistance to blackspot bruise was verified. Thirty tubers of 25 varieties and hybrids of Solanum tuberosum L. were evaluated using electronic pendulum MIDAS 88P. The analysis of variance showed a significant difference among the individual varieties and hybrids and among years as well. An evaluation scale was established for the assessment of potato genotype (genetic resource) resistance to blackspot bruise. Varieties and hybrids were included in the new designed evaluation scale in scores of 7 (high resistance) up to 9 (very high resistance), which could be regarded...
Characterisation of resistance genes resources against late blight available for Czech potato breeding by means of selected DNA markers
P. Sedlák, P. Vejl, M. Melounová, P. Křenek, J. Domkářová, J. Zoufalá
Plant Soil Environ., 2005, 51(2):82-86 | DOI: 10.17221/3559-PSE
Marker assisted selection (MAS) in potato breeding is the most developing area at present time. Methods of DNA markers are developed in all top world potato research institutes and universities oriented on plant production. This paper presents results obtained from the testing of gene resources encompassing different resistance genes against Phytophthora infestans potentially exploitable for Czech potato breeding. Three different DNA markers were studied that are linked to R1 locus in the potato chromosome V operating as a resistance against some races of P. infestans. Markering capability and frequencies of these...
The effect of plant growth regulators and chlorsulfuron on electrophoretic profiles of soluble proteins, polypeptides and antioxidant enzymes in maize seedlings
D. Nedeva, S. Petkova, Y. Angelova, L. Iliev
Plant Soil Environ., 2005, 51(2):87-93 | DOI: 10.17221/3560-PSE
Investigations were carried out with maize (Zea mays L.) seedlings. The effect of chlorsulfuron (CHF) on the electrophoretic profiles of the total and thermostable proteins, polypeptides and some antioxidant enzymes was compared to the effect of abscisic acid (ABA), benzylaminopurine (BAP) and N1-(2-chloro-4-pyridyl)-N2-phenylurea (4PU-30) applied in concentrations that inhibit the growth of seedlings. It was established that the effect of CHF on seedling growth as well as on the studied biochemical parameters was most significant. The reduction of growth by CHF was accompanied by a significant...
The trnL-F plastid DNA characters of three Poa pratensis (Kentucky bluegrass) varieties
S.D. Stoneberg Holt, L. Horová, P. Bureš, J. Janeček, V. Černoch
Plant Soil Environ., 2005, 51(2):94-99 | DOI: 10.17221/3561-PSE
The characterization of crop cultivars (varieties) will come to depend increasingly on molecular characters in addition to traditional morphological and agronomic characters. Three cultivars of Kentucky bluegrass (Poa pratensis L.), developed by the Plant Breeding Station Hladké Životice (PBHŽ), were characterized using sequences and PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) patterns from the non-coding plastid trnL-F region (trnL intron, 549 bp, and trnL-trnF intergenic spacer [IGS], 344 to 364 bp). These characters could be readily and repeatably determined not only...