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Results 121 to 150 of 1007:

Short-term effects of tillage and leaf mulch on soil properties and sunflower yield under semi-arid conditionsOriginal Paper

Koena Rivonia Selolo, Jestinos Mzezewa, Jude Julius Odhiambo

Plant Soil Environ., 2023, 69(2):55-61 | DOI: 10.17221/160/2022-PSE

A study was conducted at the University of Venda Experimental Farm, Limpopo province, South Africa to determine the effects of tillage and mulching on selected soil properties, and yield of sunflower (Helianthus annuus L.). The experiment was laid out in a split plot design with three replications during the 2018/19 and 2019/20 cropping seasons. Treatments consisted of conventional tillage (CT) and minimum tillage (MT) and three levels of avocado leaf mulch (0, 6 and 12 t/ha). Bulk density (BD), aggregate stability (AS), infiltration rate (IR), soil water content (SWC) and grain yield were determined. Tillage had no significant effect on BD in either season but influenced SWC and sunflower grain yield. CT recorded a significantly higher AS than MT during 2018/19 cropping season. Tillage × mulch interaction was significant during 2018/19 season with CT at 12 t/ha mulch recording higher AS than the MT. IR was significantly influenced by tillage × mulch interaction in both seasons with MT recording higher IR than the CT during 2018/19. Avocado mulch had no significant effect on sunflower grain yield in either season but influenced SWC in 2019/20 season. It was concluded that avocado mulch could be a relevant component of conservation agriculture but long-term studies are needed to validate the benefits observed in this study.

Ferric oxide nano-priming enhances photosynthetic and physicochemical properties of sunflower (Helianthus annuus L.) microgreensOriginal Paper

Aayushi Gupta, Rohit Bharati, Jan Kubes, Pavla Vachova, Daniela Popelkova, Lovely Mahawar, Marek Zivcak, Xinghong Yang, Marian Brestic (ORCID: 0000-0003-34

Plant Soil Environ., 2024, 70(11):702-711 | DOI: 10.17221/272/2024-PSE

In modern agriculture, nano-priming represents an innovative approach, harnessing the power of nanotechnology to enhance crop yields and nutrition. However, to effectively harness the potential of nanoparticles (NPs) for agriculture applications, understanding their mode of action and optimal application rates for positive effects on microgreen growth and physiology is critical. In this interdisciplinary study, we investigated the priming of sunflower seeds with a range of concentrations (25, 50, and 100 mg/L) of ferric oxide (Fe2O3) nanoparticles (FeNPs) and compared them with control samples. Our findings revealed a significant increase in plant biomass, leaf size, and photosynthetic activity in treated samples. The activities of photosystems I and II increased with higher FeNPs concentration. The treated samples exhibited elevated levels of total phenolics, anthocyanin, and antioxidant enzyme activity, along with increased macronutrients and micronutrients. These findings highlight the potential of FeNPs as a promising tool for enhancing plant growth and physiology in sunflower microgreens.

Agricultural waste-based lactic acid production by the fungus Rhizopus oryzae: a tool for sustainable polylactic acid production for agricultural use – a reviewReview

Chandra Sekhar Paul, Luka Stefanovic, Tatiana Robledo-Mahón, Filip Mercl, Jiřina Száková, Pavel Tlustoš

Plant Soil Environ., 2024, 70(12):739-750 | DOI: 10.17221/416/2024-PSE

Lactic acid has gained considerable attention globally due to its multi-purpose application. Commercial lactic acid production uses the fungal species Rhizopus oryzae, which produces other organic acids. A crucial point of effective fungal organic acid production is matching the fungal strains’ requirements, where the carbon source plays a major role. The highest production rate is achieved when glucose is used as a carbon source. Alternatively, we can apply carbon-rich agricultural residues as carbon sources. Using agricultural waste for lactic acid production provides a sustainable and cost-effective feedstock but also helps to reduce greenhouse gas emissions by diverting waste from landfills and decreasing reliance on fossil fuels. Moreover, polylactic acid (PLA) produced from lactic acid monomers can occur in numerous agricultural applications. We should delve deeper into sustainable methods of using carbon residues to recycle waste, foster the circular economy, and advance sustainable agriculture. Therefore, there is a need for further research on the commercial use of agricultural and food industry wastes for lactic acid production.

Effects of microplastics on farmland soils and plants: a reviewReview

Minhua Zhang, Weiguan Li, Qingkui Li, Aneela Younas, Muhammad Shaaban, Yuyang Li, Jing Liu, Yanfang Wang, Zhanqiang Ma, Zhaoyong Shi, Hongtao Shen, Ling Liu

Plant Soil Environ., 2025, 71(12):829-848 | DOI: 10.17221/180/2025-PSE

Microplastics (MPs) are plastic particles smaller than 5 mm in size, which are widely present and have become one of the major pollutants in the natural environment, and are increasingly recognised as emerging pollutants in agricultural ecosystems. Due to their small size and high mobility, MPs can easily migrate into farmland soils and attach to plant surfaces, thereby altering the physical, chemical and microbial properties of the soil. These changes may affect seed germination, plant growth, and physiological and biochemical functions. This review systematically synthesises current research on the impact of MPs on agricultural soil, focusing on their effects on soil structure, chemical properties and microbial diversity. The positive and negative effects of MPs on plant seed germination, growth, and physiological and biochemical processes are critically analysed. Furthermore, the potential ecological risks of MPs to soil and plant health are discussed. Mitigation strategies and future research priorities are proposed to address MPs contamination in agricultural systems. This study aims to provide both theoretical insights and practical references to support the prevention and control of MPs pollution in farmland soils, thereby contributing to sustainable agricultural development and soil ecosystem resilience.

Identification of nitrification inhibition in maize to mitigate soil nitrogen lossOriginal Paper

Azizah Firdausi Nur, Purwanto Benito Heru, Tawaraya Keitaro, Rachmawati Diah

Plant Soil Environ., 2023, 69(8):387-399 | DOI: 10.17221/148/2023-PSE

Nitrogen dynamics in soil produce N2O emissions. To decrease N2O emissions and conserve N, recent studies have focused on chemicals derived from root exudates that inhibit nitrification. However, selective plant breeding could be used to control nitrification activity in soil instead of fertilisers or synthetic nitrification inhibitors. In this study, we investigated the relationship between nitrification rates (NR) and related N dynamics and plant characteristics for 11 maize varieties with varying levels of nitrification inhibition (NI) compared to Brachicaria humidicola (Bh) as the positive control. In a greenhouse experiment, soil concentrations of NI, NR, NH4+-N, and NO3--N and nitrogen uptake by plants were measured. Six maize varieties had a 1.1–1.6 times lower NR than Bh. Low-NR varieties had higher NI and lower root-to-shoot ratios. NI was positively correlated with total N and shoot N content but not with cumulative N2O and NH4+-NO3-. These results show that maize has the capacity to reduce soil nitrification while increasing the total N in the soil and shoot N content in maize.

Characteristic of soil moisture utilisation with different water-sensitive cultivars of summer maize in the North China PlainOriginal Paper

Haoze Zhang, Mingliang Gao, Fuying Liu, Huabin Yuan, Zhendong Liu, Mingming Zhang, Quanqi Li, Rui Zong

Plant Soil Environ., 2024, 70(4):210-219 | DOI: 10.17221/401/2023-PSE

Summer maize cultivars are differently sensitive to soil moisture. To better understand the differences in water productivity of summer maize cultivars with different water sensitivity, a field experiment was conducted from 2020 to 2022. Three different water-sensitive summer maize cultivars were selected, including TY808 (high water-sensitive cultivar), DH605 (medium water-sensitive cultivar), and ZD958 (low water-sensitive cultivar). Soil water content (SWC), soil water storage (SWS), water consumption, water use efficiency, and grain yield were determined. The results showed that under rainfed conditions, the SWC of the medium water-sensitive cultivar DH605 in the deep soil layer was 2.1–18.2% lower than TY808 and ZD958, respectively, and the differences were significant in the 12th leaf stage (V12) and vegetative tassel stage (VT). The SWS of the high-water-sensitive cultivar TY808 was 0.7% to 6.4% higher than the other two water-sensitive cultivars from 2020 to 2022. The changes in SWS are related to the spatiotemporal distribution of precipitation. The water consumption of DH605 was higher than TY808 and ZD958 by 5.3% and 7.09% in 2020 and 2.9% and 2.8% in 2021; in 2022, DH605 is 2% higher than ZD958 and 2.8% lower than TY808, respectively. The yield of DH605 was 4.3–10.78% higher than the other two cultivars in the three-year experiment. Additionally, the 1 000-kernel weight of DH605 was the highest in TY808 and ZD958. DH605 has the highest water use efficiency, which was increased by 4.8–14.6% compared to TY808 and ZD958. Through path analysis, we found that the direct path coefficient of SWS in the VT stage on yield reached 0.999, indicating that soil moisture in the VT stage has the greatest impact on yield, followed by the blister stage (R2). In conclusion, our results suggest that the water consumption of summer maize during the VT stage is the highest, and the soil moisture condition in VT significantly affects the grain yield of summer. Planting DH605 in the North China Plain would harvest the maximum grain yield and water productivity.

Drought tolerance screening of plum rootstocks based on physiological and biochemical traitsOriginal Paper

Ines Mihaljević, Marija Viljevac Vuletić, Vesna Tomaš, Dominik Vuković, Zvonimir Zdunić

Plant Soil Environ., 2025, 71(1):1-11 | DOI: 10.17221/516/2024-PSE

Drought-tolerant rootstocks with better performance regarding water deficit is important for sustaining orchard productivity, especially in regions where water availability is unpredictable. By selecting appropriate rootstocks, fruit growers can mitigate the adverse effects of insufficient water on yields. However, the response of specific rootstocks to drought remains unknown. Our study examined the drought tolerance of five plum rootstocks (Wavit, Torinell, Adesoto, Penta, and St. Julien) focusing on their physiological and biochemical responses. To assess their tolerance under drought conditions, we evaluated leaf relative water content (RWC), chlorophyll fluorescence, lipid peroxidation, hydrogen peroxide (H2O2), proline, and phenolic content. The results showed that Torinel exhibited the highest performance index (PIABS), maximum PSII photochemical efficiency (Fv/Fm), RWC, lowest lipid peroxidation and H2O2 during the drought-stress condition. Based on our results, we identified Torinel as a rootstock with a great ability to withstand drought, suggesting that it could be applied in the breeding program to increase plum resistance to drought. The study provides insights into the drought tolerance of different plum rootstocks, identifying which ones are better suited for cultivation in water-limited environments.

Soil organic carbon characteristics affected by peanut shell biochar in saline-sodic paddy fieldOriginal Paper

Wenhao Zhu, Cuilan Li, Shun Zhou, Yan Duan, Jingjing Zhang, Feng Jin

Plant Soil Environ., 2022, 68(2):108-114 | DOI: 10.17221/426/2021-PSE

Biochar exhibits a profound impact on soil organic carbon (SOC) turnover and dynamics, but the underlying mechanism under field conditions is still unclear. A three-year field experiment was performed to evaluate the impact of peanut shell biochar applied at rates of 0, 33.75, 67.5, and 101.25 t/ha (referred to as B0, B1, B2, and B3, respectively) on SOC content and chemical composition in a saline-sodic paddy field using stable carbon isotope composition and 13C nuclear magnetic resonance technology. With increasing rates of biochar, SOC and aromatic carbon contents and alkyl carbon/oxygen-alkyl carbon and hydrophobic carbon/hydrophilic carbon ratios increased, while alkyl carbon and oxygen-alkyl carbon contents and aliphatic carbon/aromatic carbon ratio decreased. The new carbon from biochar and rice residues accounted for 26.5% of SOC under B0 and increased to above 80.0% under B2 and B3. The decay rate of old carbon was faster in biochar-amended than in unamended soil. SOC content was positively correlated with alkyl carbon/oxygen-alkyl carbon and hydrophobic carbon/hydrophilic carbon ratios but negatively correlated with aliphatic carbon/aromatic carbon ratio. The results suggest that biochar can increase SOC content by increasing its humification, aromaticity, and hydrophobicity. However, negative priming is not the main mechanism for SOC accumulation during the short-term period.

Revitalising subalpine grasslands: floristic shifts under renewed grazingOriginal Paper

Marie Mrázková-Štýbnarová, Michaela Kolářová, Radek Štencl, Prokop Šmirous, Marie Bjelková, Josef Holec, Martin Vašinka, Luděk Tyšer, Oldřich Látal

Plant Soil Environ., 2025, 71(5):338-352 | DOI: 10.17221/12/2025-PSE

The species diversity of the unique flora in the Hrubý Jeseník Mountains is currently threatened due to the absence of traditional grazing, which was historically used as a management practice. This study evaluates changes in floristic composition in areas near the Švýcárna and Ovčárna lodges, where cattle and sheep grazing was reintroduced in 2012 and 2014, respectively, after long-term abandonment. The floristic composition was assessed using permanent plots and analysed statistically. In total, 84 plant species were recorded in the Švýcárna experimental area over 12 years. All experimental plots throughout the study observed an increase in species richness. In the Ovčárna area, a similar trend was detected, particularly in grazed grasslands dominated by Avenella flexuosa, Festuca supina, and Ligusticum mutellina. The reintroduction of grazing in these areas serves not only as a symbolic return to traditional land use but primarily as an effective management tool to suppress ecological succession and maintain or enhance plant species diversity in biologically valuable habitats.

Adsorption of nicosulfuron herbicide in the agricultural soils of Bosnia and HerzegovinaOriginal Paper

Amer Sunulahpašić, Siniša Mitrić, Dragana Šunjka, Mirjana Žabić, Tihomir Predić, Milan Šipka, Luka Rodić

Plant Soil Environ., 2020, 66(4):162-166 | DOI: 10.17221/80/2020-PSE

In this study, the sorption characteristics of nicosulfuron herbicide in soils from different agricultural regions of Bosnia and Herzegovina, as well as factors influencing the sorption process, were evaluated. The analysis was performed using a batch equilibrium method. The obtained results showed that soils in Bosnia and Herzegovina are very versatile in terms of their characteristics. The Freundlich adsorption coefficient (Kf) coefficient ranged from 0.027 to 7.388, while the slope of the Freundlich isotherm (1/n) varied from 0.291 to 1.927. In soils with pH 4.31-7.60, 1/n was found to be less than 1 (0.337-0.547), and for the extremely alkaline soil with pH 8.2, 1/n was 1.927. Adsorption of nicosulfuron in the tested soils of Bosnia and Herzegovina was significantly correlated with the sand and silt content in the soil. Multiple linear regression correlating log Kf with the sand and silt content in the studied soils was also statistically significant (R2 = 0.951; P = 0.0108). The results of this study indicate that in sandy soils, which are slightly or moderately alkaline, the adsorption of nicosulfuron is very low and only a small amount of nicosulfuron will be adsorbed.

Effects of exogenous 2, 4-epibrassinolide and its inhibitors on the seed setting and yield of Tartary buckwheatOriginal Paper

Peiuyn He, Huangdanni Ran, Kaifeng Huang

Plant Soil Environ., 2025, 71(3):161-170 | DOI: 10.17221/669/2024-PSE

The aim of this study was to evaluate the effects of exogenous 2, 4-epibrassinolide (EBR) on the yield of Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.). A 2-year field experiment was conducted on Tartary buckwheat (cv. Jinqiao 2) with different concentrations (0, 0.1, 0.5, 1.0, and 2.0 mg/L) of EBR and brassinolide (BRZ, inhibitor of brassinolide synthesis). The seed setting rate, agronomic traits, and yield initially increased and then decreased with an increase in the EBR application rate. The seed setting rate, agronomic traits, and yield decreased gradually with an increase in BRZ concentration, and yield was the lowest at 2.0 mg/L. The appropriate application of exogenous EBR could promote the increase of Tartary buckwheat yield. Compared with 0 mg/L (control), the 0.1, 0.5, and 1.0 mg/L treatments increased yield by 13.53, 32.73, and 7.08%, respectively, while the high-concentration treatment (2.0 mg/L) decreased by 4.13%. In conclusion, the appropriate concentration of EBR treatment (0.5 mg/L) delayed the senescence of Tartary buckwheat by increasing its root activity and the activity of antioxidant enzymes in leaves. Simultaneously, it increased the chlorophyll content of Tartary buckwheat leaves, enhanced photosynthesis, increased nonstructural carbohydrate content, and augmented the "source," increasing the seed setting rate and yield of Tartary buckwheat. This concentration is recommended for use in the production of Tartary buckwheat.

Effects of different mulching measures on soil physicochemical properties and phosphorus fractions in orchards in the southeast hilly region of ChinaOriginal Paper

Bangning Zhou, Heming Li, Zuopin Zhuo, Lei Wang, Maojin Yang, Jinshi Lin, Fangshi Jiang, Yanhe Huang, Yue Zhang

Plant Soil Environ., 2025, 71(3):171-184 | DOI: 10.17221/675/2024-PSE

Soil phosphorus plays an important role in the soil ecological environment and sustainable development of the fruit industry in the soil hilly region of southern China, but the impact of different mulching measures on soil available phosphorus and phosphorus fractions in orchards remains unclear. In this study, soil basic physicochemical properties, available phosphorus, inorganic phosphorus fractions and their interrelationships under natural grass cover (NG), film mulch (FM) and clean tillage (CK) in orchards were explored. Compared to CK treatment, both FM and NG treatments have been shown to increase the contents of soil organic carbon (SOC), total nitrogen (TN), and available nitrogen (AN). Additionally, compared with the FM treatment, the NG treatment increased total phosphorus (TP), total potassium (TK), available potassium (AK), and soil acid phosphatase (S-ACP), resulting in greater improvements in soil fertility. The NG treatment increased the contents of aluminium-bound phosphate (Al-P) and iron-bound phosphate (Fe-P) in the 0–40 cm soil layer, whereas the FM treatment decreased the contents of Fe-P and Al-P and increased the content of occluded phosphate (O-P). Compared with the CK treatment, the NG treatment significantly increased the available phosphorus in the 0–40 cm soil layer, whereas the FM treatment significantly decreased it. Redundancy analysis revealed that pH and S-ACP were the main factors affecting soil phosphorus components. Al-P, Fe-P, and S-ACP were the three factors with the highest correlations with available phosphorus. However, according to multiple stepwise analyses, only Al-P was directly related to available phosphorus. Overall, in the southeast hilly orchards, the NG treatment improved soil nutrient and enzyme activity and is considered an effective strategy to increase the biological effectiveness of phosphorus while reducing leaching losses.

Increased uptake and accumulation of phosphorus and other nutrients by legumes enhance their bioavailability for non-legume speciesOriginal Paper

Peltier Aguiar, Margarida Arrobas, Manuel Ângelo Rodrigues

Plant Soil Environ., 2025, 71(6):409-425 | DOI: 10.17221/85/2025-PSE

Legumes are promoted in agroecosystems for their ability to fix atmospheric nitrogen (N), thereby reducing or eliminating the need for N fertilisation while also contributing N-rich organic residues, which non-legume species can subsequently utilise. In phosphorus (P)-poor soils, certain legumes appear to access less available forms of P, converting them into organic P and facilitating its use by non-legume species. This study evaluated seven legume species/cultivars and one grass species (as a control) in a trial conducted in low-fertility soils under four different growing conditions (location × year). The objective was to investigate the role of legumes in P and other nutrient uptake and accumulation in plant tissues. Some lupins and broad beans accumulated up to 30 kg/ha of P in their biomass, even without accounting for P in the roots. Calcium (Ca) and magnesium (Mg) concentrations in plant tissues were also significantly higher in legumes than in grass. In addition to concentrating certain nutrients in their tissues, legumes produced substantially more biomass due to their access to atmospheric N, resulting in considerably higher nutrient accumulation. Ca and Mg in some legumes exceeded 100 and 40 kg/ha in aboveground biomass, respectively, whereas in grasses, they remained below 4 kg/ha. Thus, when legumes are cultivated as green manure, these nutrients are returned to the soil in organic form, which can subsequently become available to non-legume crops through the mineralisation process of the organic substrate. Therefore, cultivating legumes not only enhances N availability for other species but also improves the cycling of other essential nutrients.

The effect of hydrogel and precipitation-thermal conditions on the yield and content of antinutritional compounds in potatoOriginal Paper

Krystyna Zarzecka, Marek Gugała, Iwona Mystkowska, Emilia Rzążewska

Plant Soil Environ., 2026, 72(3):165-171 | DOI: 10.17221/16/2026-PSE

A field experiment was conducted with potatoes to examine the effects of hydrogel application and weather conditions on total tuber yield and the content of potentially harmful compounds – glycoalkaloids and nitrates. The first experimental factor comprised three table cultivars: Lawenda, Rima and Provita. The second factor consisted of three treatments: the application of the hydrogel AgroNanoGel Basic at 60 and 90 kg/ha, and a control treatment without hydrogel. Statistical analysis demonstrated significant effects of cultivar, hydrogel application rates, and hydrothermal conditions in the study years on potato tuber yield. The highest yields were produced by cv. Lawenda, and the most favourable yield-forming effects were observed when the hydrogel had been applied at 90 kg/ha. The levels of antinutritional compounds were significantly affected by the experimental factors and weather conditions during the study years. Cv. Rima accumulated the lowest levels of glycoalkaloids, whereas cv. Lawenda contained the least nitrates (V). The hydrogel increased the content of both glycoalkaloids and nitrates relative to the control treatment, although their levels posed no risk to human health. Higher concentrations of antinutritional compounds were recorded in the dry and warm 2024 season than in the cooler and more humid 2025 season.

Combined effect of nitrogen and phosphorous fertiliser on nitrogen absorption and utilisation in riceOriginal Paper

Yating Zheng, Hong Chen, Guotao Yang, Rudan Wang, Nabi Farhan, Chong Li, Cheng Liang, Kaiqin Shen, Xuechun Wang, Yungao Hu

Plant Soil Environ., 2023, 69(1):25-37 | DOI: 10.17221/420/2022-PSE

The objectives of this study were to investigate the nitrogen (N) and phosphorus (P) balance fertilization strategy in paddy fields, and to evaluate the effects on N uptake and utilization in rice. In 2017-2018, the experiment was conducted using Deyou4727 hybrid rice with four different P fertilizer levels (0, 30, 60, and 90 kg/ha), marked as P0, P1, P2, P3 in turn, and four different N levels (0, 90, 150, and 270 kg/ha), similarly marked as N0, N1, N2, N3 in turn. The results showed that in the N-insufficient (N0, N1) environments, the P1 treatment increased N uptake and promoted transfer to the grain. However, high-P (P3) application increased the dry matter accumulation than other P levels, but limited the production and translocation of dry matter to some extent. In N-sufficient (N2, N3) environments, P2 level increased crop yield and N use efficiency by 11.35% and 37.01%. Unlike P2, none-P (P0) and high-P levels decreased rice dry matter translocation and transport capacity, which further affected N uptake and utilization in N-sufficient environments. Overall, the combination of the N application rate of 90 kg/ha and P application rate of 30 kg/ha, N application rate of 150, 270 kg/ha, and P application rate of 60 kg/ha had a high yield; strong nutrient accumulation and transfer ability. It was more inclined to balance N and P, which was beneficial to plant N absorption and utilization.

Occurrence of herbicides and their transformation products in sewage sludge: a reviewReview

Madhusha Mihirani Subasinghe, Filip Mercl, Sreynet Nang, Altyn Taisheva, Pavel Tlustoš

Plant Soil Environ., 2025, 71(12):849-859 | DOI: 10.17221/485/2025-PSE

The beneficial reuse of sewage sludge in agricultural soils is limited by the accumulation of micropollutants of emerging concern, which may pose significant environmental and human health risks. This review summarises recent advances in understanding the occurrence, persistence, and fate of herbicides and their transformation products in sewage sludge. Data from various geographic regions are discussed, with a focus on implications for the safe reuse of biosolids in agriculture. Most available studies have been conducted in European Union countries, where land application of biosolids is a common practice. Twelve groups of herbicides and their transformation products have been identified in sewage sludge, including glyphosate and aminomethylphosphonic acid (AMPA), phenylureas, phenoxy acids, chloroacetamides, triazines and their metabolites, triazinones, phenylcarbamates, isoxazolidinones, benzoic acids, dinitroanilines, benzofurans, phenyl ethers, and other herbicides. Among these, triazines and their metabolites were the most frequently detected, with concentration ranges of 0.01–277 ng/g and not detected (n.d.)–237 ng/g, respectively. Glyphosate and AMPA were found at particularly high concentrations (n.d.–35 000 ng/g). Phenylurea herbicides (e.g., diuron and isoproturon) were detected in a limited number of studies, with concentrations ranging from not detected to 102 ng/g. Substantial concentrations of phenoxy herbicides (2,4-d, 2,4-db, and 2,4,5-t) were also reported in sewage sludge, ranging from 50.5–864 ng/g. The available scientific literature on the occurrence of herbicides in sewage sludge focuses mainly on older, often already banned compounds, while data on currently approved herbicides remain scarce. This review highlights the need for more comprehensive global assessments of herbicides and their transformation products in sewage sludge to ensure the safe agricultural use of biosolids and minimise risks to plants and other organisms. The current lack of systematic monitoring and documentation represents a critical knowledge gap in evaluating environmental exposure and associated risks.

Optimising plastic-film mulching under drip irrigation to boost maize productivity through enhanced water and fertiliser efficiency in sub-humid regionsOriginal Paper

Xiaodong Bo, Fuqi Yao

Plant Soil Environ., 2025, 71(7):509-523 | DOI: 10.17221/213/2025-PSE

Global food security is increasingly threatened by the vulnerability of agricultural systems to climate variability, especially in sub-humid regions. Northeast China, a major maize-producing region, experiences low spring temperatures and erratic rainfall, which have prompted the widespread adoption of plastic-film mulching (PFM) combined with drip irrigation. However, systematic evaluations of how different PFM patterns affect crop productivity and resource use efficiency remain limited. This study systematically evaluated three PFM strategies – full ridge-furrow mulching (FM), ridge mulching (RM), and no mulching (NM) – in combination with 240 kg N/ha and a zero-nitrogen control under drip irrigation to determine their effects on maize (Zea mays L.) yield, water use efficiency (WUE), and nitrogen utilisation. Field experiments over two consecutive growing seasons assessed crop growth, dry matter (DM) accumulation, nitrogen dynamics, grain yield, and related efficiency parameters. Both FM and RM significantly enhanced early maize growth. At the seedling stage, FM and RM increased plant height by 43.0% and 40.1%, and leaf area index (LAI) by 141.4% and 120.4% over NM, respectively. During the same stage, DM accumulation increased by 228.9% (FM) and 224.9% (RM). These improvements reflected favourable soil hydrothermal conditions under PFM. Before heading, PFM treatments increased pre-anthesis DM accumulation by up to 19.6%, and at maturity, FM and RM raised DM by 6.1% and 5.1% over NM. PFM significantly improved grain nitrogen accumulation, with FM and RM increasing it by 31.0% and 26.9% over NM, respectively, and nitrogen harvest index (NHI), with FM and RM increasing it by 6.8% and 6.1% over NM, indicating enhanced nutrient translocation to grain. PFM also improved grain yield, with FM and RM increasing it by 15.0% and 13.5%, WUE by 17.2% and 15.7%, and nitrogen partial productivity by 16.8% and 14.1%. No significant differences in yield or WUE were observed between FM and RM. Fertilisation consistently enhanced these benefits without changing the relative efficiency ranking of treatments. Notably, the advantages of mulching diminished after the heading stage as temperature and rainfall increased. PFM (both FM and RM) under drip irrigation improves maize yield, water use, and nitrogen efficiency in sub-humid regions. This integrated practice offers a scalable and sustainable strategy to increase maize productivity and resource efficiency, supporting food security in regions facing similar climatic challenges.

Spectral characteristics of leaves diffuse reflection in conditions of soil drought: a study of soft spring wheat cultivars of different drought resistanceOriginal Paper

Dmitriy V. Rusakov, Elena V. Kanash

Plant Soil Environ., 2022, 68(3):137-145 | DOI: 10.17221/483/2021-PSE

Quick and accurate nondestructive methods of water deficiency detection prior to the appearance of visible symptoms of plant deterioration as well as estimation of photosynthesis parameters are needed to effectively control conditions of plant growth, to manage crop productivity and to implement programs of "smart farming". The aim of our investigation was to analyse spectral characteristics of leaves diffuse reflection as evident in soft spring wheat cultivars (Triticum aestivum L.) of different drought resistance in optimal conditions and under the impact of soil drought; another objective was to determine the reflection indices that could serve as criteria in the phenotyping of genotypes according to their photosynthetic apparatus capacity and the efficiency of light use as well as in the forecasting of genotypes potential productivity and their drought resistance. Wheat plants of 4 drought-resistant and 4 non-resistant cultivars were grown under controlled conditions in the protected ground. In the vessels with simulated soil drought, the moisture content was 30% of total field capacity, while in the control sample it was 80%. Spectral characteristics of radiation reflected from the leaf surface were recorded with the spectrometer HR2000, and then reflection indices were calculated whose value is closely related to the activeness of the photosynthetic apparatus. The experiments conducted showed that in the system of interaction between the soil, the plant and the effective layer of the atmosphere all analysed diffuse reflection indices changed with the emergence of water deficit. The index of photosynthetic apparatus capacity (ChlRI) is less susceptible to short-term soil drought than the indices of the efficiency of light use in the process of photosynthesis (R800, photochemical reflection index (PRImod) and flavonoid index (FRImod)) which change significantly, so that the degree of their change may be a reliable enough indicator of plant stress caused by water deficiency. It is advisable, however, when estimating and comparing the reaction of various plant cultivars, lines and new forms to the developed water deficiency, to include in the array of plants examined those cultivars whose optical properties and the range of their variation resulting from water deficit are known. This will ensure a more reliable ranking of analysed genotypes according to their drought resistance and will enhance the accuracy of the diagnosis.

Enhanced maize yield and nitrogen efficiency with low molecular weight fulvic acid: insights into chlorophyll a/b ratio and nitrogen metabolising enzyme activityOriginal Paper

Fugui Li, Shujie Zhang, Longhang Chai, Zhiqiang Guo, Peipei Li, Yanlai Han, Yi Wang

Plant Soil Environ., 2024, 70(10):632-643 | DOI: 10.17221/320/2024-PSE

This study investigates the impact of various molecular weights (MWs) of fulvic acid (FA) on maize growth, grain yield, and nutrient uptake under different nitrogen levels (NLs). A 2 × 3 balanced design was employed, with high (0.2 g N/kg) and low (0.05 g N/kg) NLs, and three FA MW ranges (W1 ≤ 3 000 D, 3 000 < W2 ≤ 10 000 D, W3 > 10 000 D) were applied at 25 mg/kg in soil. Significant interactions between NLs and FA MWs were observed in chlorophyll a/b ratio, nitrate reductase and glutamate dehydrogenase activities, nitrogen content, and nitrogen uptake efficiency. Overall, under different NLs, FA application reduced chlorophyll a/b ratio, increased nitrogen metabolism enzyme activities, promoted maize growth, and thereby improved grain yield and nitrogen fertiliser uptake efficiency. Additionally, the promotion effect of low MW FA on these indicators outweighed that of high MW FA, yet the latter exhibited a more pronounced effect on increasing grain nitrogen concentration. Structural equation model analysis revealed direct effects of chlorophyll content, nitrogen accumulation, nitrogen uptake efficiency, NLs and FA MWs on maize grain yield, with FA MWs negatively impacting yield.

Biotransformation of food waste into biofertilisers through composting and anaerobic digestion: a reviewReview

Nurul Solehah Mohd Zaini, Abedelazeez J.D. Khudair, Aliah Zannierah Mohsin, Elicia Jitming Lim, Wakisaka Minato, Hamidah Idris, Jamilah Syafawati Yaacob, Muhamad Hafiz Abd Rahim

Plant Soil Environ., 2023, 69(9):409-420 | DOI: 10.17221/101/2023-PSE

A growing world population means greater pressure on earth’s resources. Currently, 30% of food is wasted, which poses a significant risk to both humans and the environment. One way to offset the growth in food waste (FW) is through the process of microbial bioconversion, whereby FW is transformed into a range of nutrient-dense biofertilisers. This approach not only promotes a highly desirable circular economy, but it can also reduce the use of inorganic fertilisers, which adversely impact the environment through increased greenhouse gases, changes in soil and water characteristics, and loss of biodiversity. The bioconversion of FW to biofertiliser relies on the processes of aerobic (composting) and anaerobic digestion. Recently, alternative decomposition techniques included growing specific beneficial microbes, such as effective microorganisms, to speed up the breakdown process. Microorganisms can act as biostimulants and biodecomposers, possessing nutrient-fixing abilities and providing protection from biotic and abiotic stresses, thus enhancing plant growth and overall health. The potential uses of FW are complex and diverse, but research is actively done to effectively utilise this resource for biofertiliser applications.

Assessment of carbon sequestration as affected by different management practices using the RothC modelOriginal Paper

Jakub Prudil, Lubica Pospíšilová, Tamara Dryšlová, Gabriela Barančíková, Vladimír Smutný, Luboš Sedlák, Pavel Ryant, Petr Hlavinka, Miroslav

Plant Soil Environ., 2023, 69(11):532-544 | DOI: 10.17221/291/2023-PSE

Long-term field experiments provide a valuable dataset for predicting changes in soil organic carbon (SOC) stocks in different agricultural systems. The RothC-26.3 model was used to simulate changes in SOC in the monoculture of spring barley (Hordeum vulgare L.) and the Norfolk crop rotation during 1972–2100. The potential of the Gleyic Fluvisol Clayic to sequester organic carbon was investigated. The studied soil was heavily textured, with medium organic carbon content. Four management scenarios in the monoculture and six management scenarios in the Norfolk crop rotation were evaluated. Three different global climate models (MPI, MRI, CMSS) representing the uncertainty of future climate conditions were used. Results showed that carbon stocks were mainly influenced by plant residue inputs and exogenous organic materials application. The projection showed trends of carbon stocks decreasing in the case of monoculture management. Results also documented that management scenario D with straw incorporation and intercrops represented sustainability and carbon stock increase during all modelled climate scenarios. The SOC stock at the end of the century was approximately 66 t/ha. This represents a moderate sequestration of SOC of approximately 0.09 t/ha/year.

Geographical variations in soil properties and bacterial community diversity across major lavender (Lavandula angustifolia Mill.) cultivation regions in the Ili River ValleyOriginal Paper

Junnan Jian, Shimin Tang, Shengjun Zhang, Ling Zhu, Bian Ran, Xuechao Zhang, Weikang Luo, Shilei Dong

Plant Soil Environ., 2025, 71(11):805-819 | DOI: 10.17221/377/2025-PSE

The Ili region hosts China’s largest lavender cultivation base, yet soil bacterial diversity in its primary cultivation areas remains understudied. To address this, we compared soil bacterial communities across four major cultivation counties (Chabuchar, Agricultural Research Institute, Yining, and Huocheng). Essential oil profiles, soil properties, and bacterial community characteristics were analysed to elucidate microbial variations and environmental interactions. The results showed that: (1) The essential oil yield (1.14%) and linalool content (41.04%) in the Huocheng County cultivation area were significantly higher than those in other areas, and the essential oil quality was relatively the best; (2) the soil bacterial communities in different main cultivation areas shared certain commonalities. At the phylum level, Proteobacteria, Acidobacteriota, Gemmatimonadota, and Actinobacteriota were the dominant phyla, and their relative abundances varied by region and soil layer, and (3) the redundancy analysis results showed that soil bacterial communities were comprehensively affected by environmental factors such as pH, total nitrogen, total phosphorus, soil organic carbon, longitude, and altitude. The significant positive correlations between the abundance of Vicinamibacteraceae (Acidobacteriota) in Huocheng County soils and both soil total phosphorus and linalool content suggest a putative mechanism whereby this bacterial taxon enhances lavender terpenoid synthesis by facilitating phosphorus cycling. Overall, these results suggest that geographically driven climatic variations dynamically alter the soil bacterial community, thereby influencing lavender growth and the final essential oil quality.

Comparison of potassium quantity-intensity relationships in tropical paddy soil under tillage and no-tillage systems after fifteen growing seasonsOriginal Paper

Soni Isnaini, Maryati, A. Arivin Rivaie

Plant Soil Environ., 2023, 69(1):1-9 | DOI: 10.17221/337/2022-PSE

The information on the behaviour of potassium (K+) in tropical paddy rice soils, which is important for a better understanding of the plant availability of K+ is still very limited. We compared the quantity-intensity (Q/I) relationships for K+ under conventional tillage and no-tillage systems in tropical paddy fields in the absence and presence of K+ fertiliser in the addition of nitrogen. The results showed that the values of the activity ratio for K (ARK) and potential buffering capacities (PBCK) in the no-tillage rice field were respectively 16% and 33% higher than that in the conventional tillage field. With the addition of K fertiliser, the value of exchangeable K in equilibrium (ΔK0) in the no-tillage paddy field was 67.9% greater than that in the conventional tillage field. This indicates that K fertilisation is more efficient when applied on a no-tillage paddy field. When the K fertiliser was added (49.8 kg K/ha), the application of N fertiliser at the rate of 115 and 184 kg N/ha resulted in a higher ARK value than that at the rate of 46 kg N/ha. This suggests that the simultaneous application of K and N fertiliser was able to increase exchangeable K in the soil. The application of no-tillage increased of the dry grain yield of rice (about 10%) compared with the application of conventional tillage. Meanwhile, there were significant relationships between the rice yield with the ARK and ΔK0. Moreover, the ARK was significantly correlated with K-uptake.

The effect of soil tillage and sulphur fertilisation on content and ratios of macronutrients in the grain of spring triticaleOriginal Paper

Hanna Klikocka, Anna Podleśna, Bartosz Narolski, Janusz Podleśny

Plant Soil Environ., 2025, 71(5):308-319 | DOI: 10.17221/113/2025-PSE

The aim of the experiment was to determine the yield, content and uptake of macronutrients and their ratio in spring triticale, Milewo cultivar. The field experiment was conducted in the years 2014–2016 on Cambisols. The first experimental factor was a system of soil tillage (traditional (TRD) and reduced (RED)), and the second was sulphur fertilisation (0, 25 and 50 kg S/ha). Based on the study, it was found that the application of conventional tillage and the addition of sulphur fertilisation to NPK significantly increased spring triticale grain yield. The application of reduced tillage positively affected the increase in content and uptake in grain dry matter (DM) of N, S, P, K, Mg and Ca. Adding sulphur (S) to NPK fertilisation favourably increased the content and uptake of N, S, Mg, and Ca and did not affect the content of P and K. The application of reduced tillage expanded the ionic ratio of N : S, P : S while it narrowed the N : P ratio. However, the tillage system did not affect the ionic ratios Ca : P, K : Mg, K : (Ca + Mg) and molar K : (Ca + Mg). Adding sulphur to NPK fertilisation narrowed the N : S and P : S ratios while expanding the N : P and Ca : P ratios. Weather conditions during the 2016 growing season (relatively dry, k = 1.71) favoured spring triticale yield and uptake with dry grain weight of N, S, P, Mg and Ca. The highest N, S, P, K and Ca content in grain dry matter was shown in the 2014 season (relatively humid, k = 1.96). Numerous correlations were found between grain yield and the content, uptake and reciprocal ratios of elements in grain.

Foliar silicon modulates structural and biochemical responses of buckwheat to water deficitOriginal Paper

Jiri Krucky, Vaclav Hejnak, Pavla Vachova, Jana Ceska, Jan Kubes, Milan Skalický

Plant Soil Environ., 2026, 72(1):66-75 | DOI: 10.17221/539/2025-PSE

Drought is a major abiotic stressor that limits crop growth and is often associated with oxidative stress. We evaluated whether foliar silicon (Si) application affects primary root anatomy, plant height, and phenolic metabolism in three common buckwheat (Fagopyrum esculentum) cultivars (La Harpe, Panda, and Smuga) exposed to water deficit. Plants were grown under controlled conditions in four treatments: control; drought; control + Si, and drought + Si. Qualitative anatomical assessment revealed that Si promoted more advanced development of the primary root central cylinder, most notably in La Harpe under drought conditions, where a continuous ring of secondary xylem and a well-developed pith were observed. Drought significantly reduced plant height in all cultivars; Si partially alleviated this reduction in La Harpe and Panda, but not in Smuga. Drought generally increased total phenolic content (TPC) and phenolic acid content (PAC) in both leaves and roots, and Si further enhanced these responses, with the highest values under drought + Si. Overall, the results indicate cultivar-dependent effectiveness of foliar silicon (Si) and suggest that Si contributes to coordinated structural and biochemical adjustments under water deficit conditions. To assess the transferability of these responses, further verification across a broader range of genotypes and under different intensities and durations of drought is warranted.

Contribution to the turbidimetric method for sulphur determination in arable soilsOriginal Paper

Olya Surleva, Katrin Chavdarova, Vilyana Kazanlaklieva, Lyudmila Angelova, Andriana Surleva

Plant Soil Environ., 2025, 71(10):695-707 | DOI: 10.17221/250/2025-PSE

Sulphur (S) plays an important role in agriculture, being the fourth major contributor to improved quality of crops and increased yields. The applied methods for the estimation of different forms of S in soil aimed at assessing the sulphur availability to plants in various conditions. Nowadays, the wider spreading of regions with sulphur deficiency imposes optimisation of the soil testing procedures in order to increase their availability for laboratories. This study contributes to improving the analytical performance of the turbidimetric method in determining water-soluble sulphate in soil after leaching with the CaCl2 reagent. The modified testing protocol showed: method limit of quantification of 5.0 mg/kg; precision as relative standard deviation less than 3%; recovery of fortified soil samples 103 ± 18%. The expanded uncertainty was 2.3 mg/kg SO42–-S (K = 2, norm.). The proposed testing protocol was inexpensive, fast, used simple equipment and procedures, easily adoptable in regular laboratories, and showed characteristics suitable for the estimation of water-soluble sulfate in arable soils. A set of 546 soil samples was tested, and 74% were found to be sulphur deficient with SO42–-S < 10 mg/kg and sulfur availability index < 6.0. Thus, the availability of laboratory analysis to a broader group of farmers could contribute to effective fertilisation programs, as the newly proposed fertiliser blending technologies are based on adequate estimation of sulfur availability in arable soils.

Study on the main physicochemical characteristics of different plant cultivation substrates and their effects on standard rosesOriginal Paper

Yingguo Wang, Tingting Cao, Juan Li, Hang Zhou, Haiou Zhang

Plant Soil Environ., 2024, 70(12):799-808 | DOI: 10.17221/258/2024-PSE

Standard roses are a widely used ornamental plant in urban landscapes, valued for their attractive flowers and adaptability to various environmental conditions. This study investigated how different substrate types affect the growth and development of standard roses and their potential to improve the ecology of urban landscapes. Nine substrate conditions (rotted corn stover, decomposed shiitake mushroom residue, perlite, and combinations) were compared with field soil as a control treatment. The physical and chemical characteristics of each substrate were analysed, and the growth and development of standard rose plants were observed over six months. The results indicated that the substrate T4 (70% rotted corn stover, 15% decomposed shiitake mushroom residue, 15% perlite) achieved the highest evaluation index, leading to superior plant growth compared to other substrates. This combination provided optimal water retention, aeration, and nutrient supply, making it the most effective substrate for cultivating standard roses. Additionally, the use of these substrates can improve soil quality and reduce environmental pollution, offering a sustainable option for urban landscape management.

Lignite-derived organic fertiliser enhanced the carbon sequestration capacity of woody plant by improving soil quality and promoting plant growthOriginal Paper

Qianru Wu, Ruofan Bu, Taotao Wang, Bei Zhang, Kylan Jin, Liang Chen

Plant Soil Environ., 2025, 71(10):708-721 | DOI: 10.17221/265/2025-PSE

As essential natural carbon sinks, woody plants play a key role in urban ecological restoration. The lignite-derived organic fertiliser (LOF) may promote plant growth and carbon sequestration by improving soil properties. This study investigated LOF effects on three typical woody plants – Styphnolobium japonicum (L.) Schott. with taproots, Malus × micromalus Makino with fibrous roots, and Malus domestica Borkh. with both taproots and fibrous roots – focused on soil properties improvement during a three-year planting experiment (2021–2023). The results indicated that LOF application significantly increased soil organic matter (SOM) content, with and without woody plants, by 82.3% and 54.9%, respectively. Concurrently, LOF influenced soil microbial characteristics, especially enhancing the 16S rRNA gene copy number by 0.99 times. For plant growth, LOF application increased root length, volume, and tip number in Malus domestica Borkh. by 37.4, 27.4, and 26.0%, respectively, and in Styphnolobium japonicum (L.) Schott by 43.8, 76.7, and 26.6%, respectively. However, in Malus × micromalus Makino, while root volume increased by 3.8%, root length and tip number decreased by 10.0% and 26.9%, respectively. Additionally, the LOF application increased the soil plant analysis development (SPAD) values of woody plant leaves by 5.3%, indicating improved chlorophyll content and plant health. These findings demonstrate that LOF applications may significantly enhance soil quality and promote plant growth, contributing to improved terrestrial carbon sequestration.

Crop sequence effects on energy efficiency and land demand in a long-term fertilisation trialOriginal Paper

Gerhard Moitzi, Reinhard W. Neugschwandtner, Hans-Peter Kaul, Helmut Wagentristl

Plant Soil Environ., 2021, 67(12):739-746 | DOI: 10.17221/440/2021-PSE

The effect of crop sequences (CR - continuous winter rye; CropR - three-field crop rotation of winter rye-spring barley-bare fallow) and fertilisation systems (unfertilised control, mineral fertiliser (NPK), farmyard manure (FYM)) on crop yield, energy efficiency indicators and land demand were analysed in a long-term experiment under Pannonian climate conditions. Due to lower fuel consumption in the bare fallow, the total fuel consumption for CropR was 27% lower than in CR. It was for NPK and FYM fertilisation by 29% and 42% higher than in the control. Although the energy output was lower in CropR than CR, the energy use efficiency for grain production increased by 35% and for above-ground biomass production by 20%. Overall crop sequences, the NPK treatment had higher crop yields, energy outputs and net-energy output with a lower energy use efficiency than the unfertilised control. CropR increased the land demand just by 20% in comparison to CR, although one-third of the land was not used for crop production. The land demand could be decreased with fertilisation by 50% (NPK) or 48% (FYM). A bare fallow year in the crop rotation decreased the crop yield, energy input and increased the energy use efficiency and land demand.

Characterization of selenium accumulation of different rice genotypes in Chinese natural seleniferous soilOriginal Paper

Biaojin ZHANG, Yihua WEI, Song YAN, Huaxin SHI, Yuanyuan NIE, Guoxing ZOU, Xiangxi ZHANG, Linguang LUO

Plant Soil Environ., 2019, 65(1):15-20 | DOI: 10.17221/603/2018-PSE

In this study, the difference of Se content in brown rice of different rice genotypes was evaluated on natural seleniferous soil. Firstly, the Se content of brown rice in 80 rice germplasm resources was determined, which ranged from 0.0249-0.1426 mg/kg, showing obvious differences. Next, two cultivars with a significant difference in Se content in brown rice, i.e., cv. Wuyangeng (high) and cv. IR68144 (low), were used to study the distribution pattern of Se in different organs. Moreover, the physiological mechanism of the Se content diversity in brown rice of the two cultivars was explored preliminarily. The results showed that the Se content of cv. Wuyangeng was 2-3 times higher compared to cv. IR68144. However, the Se contents of cv. IR68144 root and leaf were significantly higher than that of cv. Wuyangeng under both natural soil environment and artificial nutrient solution culture. Cv. IR68144 also had a stronger root Se accumulation coefficient and shoot Se transfer coefficient. Consequently, it can be inferred that the stronger Se transfer ability from stem to grain was the key reason for the higher Se content in brown rice cv. Wuyangeng than in cv. IR68144.

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