Plant Soil Environ., 2002, 48(5):224-229 | DOI: 10.17221/4230-PSE

The determination of coumestrol in alfalfa (Medicago sativa) by capillary electrophoresis

J. Moravcová, T. Kleinová, R. Loučka
1 Institute of Chemical Technology, Prague, Czech Republic
2 Research Institute of Animal Production, Prague, Czech Republic

High performance capillary electrophoresis (HPCE) on an uncoated fused-silica capillary column using a borate buffer at pH 9.2 as electrolyte and diode-array detection was developed for the determination of coumestrol in alfalfa. The linear detector response was established in the concentration range 0.76-140 mg.dm-3, the minimum detectable limit was 0.39 mg.dm-3, and migration time of coumestrol was 5 min. 3-Isobutyl-1-methylxanthin was used as an internal standard. Coumestrol was isolated by acid extraction employing a mixture hydrochloric acid-acetonitrile at 95°C for 30 min followed by solid phase extraction. Relative standard deviations of reproducibility and repeatability were 1.77% and 5.49%, respectively. Spiking recovery value of 92% was achieved. Alfalfa, variety Morava, contains 148-248 mg.kg-1 coumestrol in dry matter. The proposed method is useful for routine analyses.

Keywords: phytoestrogens; alfalfa; plant analysis; animal feed

Published: May 31, 2002  Show citation

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Moravcová J, Kleinová T, Loučka R. The determination of coumestrol in alfalfa (Medicago sativa) by capillary electrophoresis. Plant Soil Environ. 2002;48(5):224-229. doi: 10.17221/4230-PSE.
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