Plant Soil Environ., 2006, 52(10):435-440 | DOI: 10.17221/3463-PSE

The effect of inoculation of pea plants with mycorrhizal fungi and Rhizobium on nitrogen and phosphorus assimilation

M. Geneva1, G. Zehirov1, E. Djonova2, N. Kaloyanova2, G. Georgiev1, I. Stancheva1
1 Department of Plant Mineral Nutrition and Water Relations, Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
2 Department of Soil Microbiology, N. Poushkarov Institute of Soil Science, Sofia,

The study evaluated the response of pea (Pisum sativum cv. Avola) to arbuscular mycorrhizal fungi (AM) species Glomus mosseae and Glomus intraradices and Rhizobium leguminosarum bv. viceae, strain D 293, regarding the growth, photosynthesis, nodulation and nitrogen fixation activity. Pea plants were grown in a glasshouse until the flowering stage (35 days), in 4 kg plastic pots using leached cinnamonic forest soil (Chromic Luvisols - FAO) at P levels 13.2 (P1) and 39.8 (P2) mg P/kg soil. The obtained results demonstrated that the dual inoculation of pea plants significantly increased the plant biomass, photosynthetic rate, nodulation, and nitrogen fixation activity in comparison with single inoculation with Rhizobium leguminosarum bv. viceae strain D 293. On the other hand, coinoculation significantly increased the total phosphorus content in plant tissue, acid phosphatase activity and percentage of root colonization. The effectiveness of coinoculation with Rhizobium leguminosarum and Glomus mosseae was higher at the low phosphorus level while the coinoculation with Glomus intraradices appeared to be the most effective at higher phosphorus level.

Keywords: Pisum sativum; Glomus mosseae; Glomus intraradices; Rhizobium leguminosarum

Published: October 31, 2006  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Geneva M, Zehirov G, Djonova E, Kaloyanova N, Georgiev G, Stancheva I. The effect of inoculation of pea plants with mycorrhizal fungi and Rhizobium on nitrogen and phosphorus assimilation. Plant Soil Environ. 2006;52(10):435-440. doi: 10.17221/3463-PSE.
Download citation

References

  1. Abd-Alla M.H. (1994): Use of organic phosphorus by Rhizobium leguminosarum biovar viceae phosphatases. Biol. Fertil. Soils, 18: 216-218. Go to original source...
  2. Al-Niemi T.S., Kahn M.L., McDermott T.R. (1997): P metabolism in the bean-Rhizobium tropici symbiosis, Plant Physiol., 113: 1233-1242. Go to original source... Go to PubMed...
  3. Bethlenfalvay G.J., Brown M.S., Franson R.L. (1990): Glycine-Glomus-Bradyrhizobium symbiosis. Plant Physiol., 94: 723-728. Go to original source... Go to PubMed...
  4. Dilworth M.J. (1974): Dinitrogen fixation. Ann. Rev. Plant Physiol., 25: 181-214. Go to original source...
  5. Duff S.M., Sarath G., Plaxton W.C. (1994): The role of acid phosphatases in plant phosphorus metabolism. Physiol. Plant., 90: 791-800. Go to original source...
  6. Giovanetti M., Mosse B. (1980): An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots. New Phytopatol., 84: 489-500. Go to original source...
  7. Hardy R.W.F., Burns R.C., Holsten R.D. (1973): Applications of the acetylene-ethylene assay for measurement of N 2-fixation. Soil Biol. Biochem., 5: 47-81. Go to original source...
  8. Ivanov P. (1984). A new acetate-lactate method for determination of soil phosphorus and potassium accessible for plants. Soil Sci. Agrochem., 19: 88-96. (In Bulgarian)
  9. Jackson N.E., Franklin R.E., Miller R.H. (1972): Effects of vesicular-arbuscular mycorrhizae on growth and phosphorus content of three agronomic crops. Soil Sci. Soc. Am. Proc., 36: 64-67. Go to original source...
  10. Jakobsen I., Rosendahl L. (1990): Carbon flow into soil and external hyphae from roots of mycorrhizal cucumber plants. New Phytol., 115: 77-83. Go to original source...
  11. Jia Y.S., Gray V.M., Straker C.J. (2004): The influence of Rhizobium and arbuscular mycorrhizal fungi on nitrogen and phosphorus accumulation by Vicia faba. Ann. Bot., 94: 251-258. Go to original source... Go to PubMed...
  12. Kolawole G.O., Kang B.T. (1997): Effect of seed sides and phosphorus fertilization on growth of select legumes. Commun. Soil Sci. Plant Ann., 28: 1223-1235. Go to original source...
  13. Lowry O., Lopez A. (1946): The determination of inorganic phosphate in the presence of labile phosphate esters. J. Biol. Chem., 162: 421-426. Go to original source...
  14. Lynch J., Lauchli A., Epstein E. (1991): Vegetative growth of the common bean in response to phosphorus nutrition. Crop Sci., 31: 380-387. Go to original source...
  15. Olivera M., Tejera N., Iribarne C., Ocana A., Liuch C. (2004): Growth, nitrogen fixation and ammonium assimilation in common bean (Phaseolus vulgaris): effect of phosphorus. Physiol. Plant., 121: 498-505. Go to original source...
  16. Rychter A.M., Randall D.D. (1994): The effect of phosphate deficiency on carbohydrate metabolism in bean roots. Physiol. Plant., 91: 383-388. Go to original source...
  17. Saxena A.K., Rathi S.K., Tilak K.V.B.R. (1997): Differential effect of various endomycorrhizal fungi on nodulating ability of green gram by Bradyrhizobium sp. (Vigna) strain S24. Biol. Fertil. Soils, 24: 175-178. Go to original source...
  18. Smith F.A., Smith S.E. (1996): Mutualism and parasitism: diversity in function and structure in the arbuscular (VA) mycorrhizal symbiosis. Adv. Bot. Res., 22: 1-43. Go to original source...
  19. Smith S.E., Reed D.J. (1997): Mycorrhizal symbiosis. London Academic Press.
  20. Tabatabai M.A., Bremner J.M. (1969): Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol. Biochem., 1: 301-307. Go to original source...
  21. Woolhouse H.W. (1975): Membrane structures and transports problems considered in relation to phosphorus and carbohydrate movements and regulation of endotrophic mycorrhizal associations. In: Sanders F.E., Mosse B., Tinker P.B. (eds.): Endomycorrhizas. London, Academic Press: 209-239.
  22. Xavier L.J.C., Germida J.J. (2003): Selective interaction between arbuscular mycorrizal fungi and Rhizobium leguminosarum bv. viceae enhance pea yield and nutrition. Biol. Fertil. Soils, 37: 262-267. Go to original source...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.