Plant Soil Environ., 2006, 52(10):435-440 | DOI: 10.17221/3463-PSE

The effect of inoculation of pea plants with mycorrhizal fungi and Rhizobium on nitrogen and phosphorus assimilation

M. Geneva1, G. Zehirov1, E. Djonova2, N. Kaloyanova2, G. Georgiev1, I. Stancheva1
1 Department of Plant Mineral Nutrition and Water Relations, Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
2 Department of Soil Microbiology, N. Poushkarov Institute of Soil Science, Sofia,

The study evaluated the response of pea (Pisum sativum cv. Avola) to arbuscular mycorrhizal fungi (AM) species Glomus mosseae and Glomus intraradices and Rhizobium leguminosarum bv. viceae, strain D 293, regarding the growth, photosynthesis, nodulation and nitrogen fixation activity. Pea plants were grown in a glasshouse until the flowering stage (35 days), in 4 kg plastic pots using leached cinnamonic forest soil (Chromic Luvisols - FAO) at P levels 13.2 (P1) and 39.8 (P2) mg P/kg soil. The obtained results demonstrated that the dual inoculation of pea plants significantly increased the plant biomass, photosynthetic rate, nodulation, and nitrogen fixation activity in comparison with single inoculation with Rhizobium leguminosarum bv. viceae strain D 293. On the other hand, coinoculation significantly increased the total phosphorus content in plant tissue, acid phosphatase activity and percentage of root colonization. The effectiveness of coinoculation with Rhizobium leguminosarum and Glomus mosseae was higher at the low phosphorus level while the coinoculation with Glomus intraradices appeared to be the most effective at higher phosphorus level.

Keywords: Pisum sativum; Glomus mosseae; Glomus intraradices; Rhizobium leguminosarum

Published: October 31, 2006  Show citation

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Geneva M, Zehirov G, Djonova E, Kaloyanova N, Georgiev G, Stancheva I. The effect of inoculation of pea plants with mycorrhizal fungi and Rhizobium on nitrogen and phosphorus assimilation. Plant Soil Environ. 2006;52(10):435-440. doi: 10.17221/3463-PSE.
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