Plant Soil Environ., 2008, 54(4):149-154 | DOI: 10.17221/387-PSE

Potentially dangerous fusarioid microorganisms associated with rot of hops (Humulus lupulus L.) plants in field culture

M. Gryndler1, K. Krofta2, H. Gryndlerová1, L. Soukupová1, H. Hršelová1, J. Gabriel1
1 Institute of Microbiology of the Academy of Science of the Czech Rrepublic, v.v.i., Prague, Czech Republic
2 Hop Research Institute Co. Ltd., Žatec, Czech Republic

Several fusarioid microorganisms were isolated as potential pathogens of hop (Humulus lupulus L.) but their virulence was not proved in inoculation trials in field conditions. Molecular search for other possible pathogens was then performed. Using terminal restriction fragment length polymorphism (TRFLP), Gibberella pulicaris (anamorph: Fusarium sambucinum) was identified as a probable cause of the hop wilting. The primary cause of the disease is wounding of hop crowns by feeding of rosy rustic moth (Hydraecia micacea) caterpillars or by defect pruning and other unfavourable circumstances. The specific primer HLf1 was designed that can be used to detect the pathogen in soil and in damaged plant tissues.

Keywords: Humulus lupulus L.; bine wilt; rootstock; DNA; artificial infection; fusarium canker; soil microflora

Published: April 30, 2008  Show citation

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Gryndler M, Krofta K, Gryndlerová H, Soukupová L, Hršelová H, Gabriel J. Potentially dangerous fusarioid microorganisms associated with rot of hops (Humulus lupulus L.) plants in field culture. Plant Soil Environ. 2008;54(4):149-154. doi: 10.17221/387-PSE.
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