Plant Soil Environ., 2008, 54(12):529-536 | DOI: 10.17221/427-PSE

Antioxidative protection in wheat varieties under severe recoverable drought at seedling stag

L. Simova-Stoilova1, K. Demirevska1, T. Petrova2, N. Tsenov2, U. Feller3
1 Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
2 Dobrudja Agricultural Institute, General Toshevo, Bulgaria
3 Institute of Plant Sciences, University of Bern, Bern, Switzerland

The antioxidative protection in leaves of four winter wheat (Triticum aestivum L.) varieties with different field drought resistance was studied under severe recoverable soil drought at seedling stage by withholding irrigation for 7 days (57-59% leaf water deficit) followed by rewatering. A 3-fold raise in electrolyte leakage and a sharp increase in proline accumulation corresponded to drought severity. Hydrogen peroxide content and catalase (CAT) activity were maintained low under stress. Peroxidase (GPX) activity increased, whereas superoxide dismutase (SOD) activity only slightly changed. The content of ascorbate and low-molecular thiols diminished under severe drought and was restored in recovery. Malondialdehyde level was not changed significantly in drought-treated plants but raised after re-watering. In recovery CAT activity became significantly higher whereas GPX activity diminished. Three isoforms of SOD, one of catalase and three of GPX were revealed. Proline accumulation had a predominant role in drought response. As for varieties, drought sensitivity or tolerance was not necessarily correlated with differences in the antioxidative response at early vegetative stage.

Keywords: antioxidative protection; drought; proline; recovery; seedlings; wheat (Triticum aestivumL.)

Published: December 31, 2008  Show citation

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Simova-Stoilova L, Demirevska K, Petrova T, Tsenov N, Feller U. Antioxidative protection in wheat varieties under severe recoverable drought at seedling stag. Plant Soil Environ. 2008;54(12):529-536. doi: 10.17221/427-PSE.
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    References

    1. Ashraf M., Foolad M.R. (2007): Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ. Exp. Bot., 59: 206-216. Go to original source...
    2. Bates L.S., Waldren R.O., Teare I.D. (1973): Rapid determination of free proline for water-stress studies. Plant Soil, 39: 205-207. Go to original source...
    3. Bradford M.M. (1976): A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254. Go to original source...
    4. Chaves M.M., Oliveira M.M. (2004): Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. J. Exp. Bot., 55: 2365-2384. Go to original source... Go to PubMed...
    5. Dalmia A., Savhney V. (2004): Antioxidant defense mechanism under drought stress in wheat seedlings. Physiol. Mol. Biol. Plants, 10: 109-114.
    6. Demirevska-Kepova K., Simova-Stoilova L., Stoyanova Z., Hölzer R., Feller U. (2004): Biochemical changes in barley plants after excessive supply of copper and manganese. Environ. Exp. Bot., 52: 253-266. Go to original source...
    7. Edreva A., Hadjiiska E. (1984): About the determination of sulfhydril (thiol) group content in plant material. Plant Physiol. (Bulg.), 10: 73-83. (In Bulgarian)
    8. Feng Z., Jin-Kui G., Ying-Li Y., Wen-Liang H., Li-Xin Z. (2004): Changes in the pattern of antioxidant enzymes in wheat exposed to water deficit and rewatering. Acta Physiol. Plant., 26: 345-352. Go to original source...
    9. Guo Z., Ou W., Lu S., Zhong Q. (2006): Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiol. Biochem., 44: 828-836. Go to original source... Go to PubMed...
    10. Hodges D.M., Andrews C.J., Johnson D.A., Hamilton R.I. (1996): Antioxidant compound responses to chilling stress in differentially sensitive inbred maize lines. Physiol. Plant., 98: 685-692. Go to original source...
    11. Hodges D.M., de Long J.M., Forney Ch.F., Prange R.K. (1999): Improving the thiobarbituric acid-reactivesubstances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207: 604-611. Go to original source...
    12. Mittler R. (2002): Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci., 7: 405-410. Go to original source... Go to PubMed...
    13. Munné-Bosch S., Lalueza P. (2007): Age-related changes in oxidative stress markers and abscisic acid levels in a drought-tolerant shrub, Cistus clusii, grown under Mediterranean field conditions. Planta, 225: 1039-1049. Go to original source... Go to PubMed...
    14. Noctor G., Foyer C.H. (1998): Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol., 49: 249-279. Go to original source... Go to PubMed...
    15. Sairam R.K., Srivastava G.S. (2001): Water stress tolerance of wheat (Triticum aestivum L.): Variations in hydrogen peroxide accumulation and antioxidant activity in tolerant and susceptible genotypes. J. Agron. Crop Sci., 186: 63-70. Go to original source...
    16. Sgherri C.L.M., Maffei M., Navari-Izzo F. (2000): Antioxidative enzymes in wheat subjected to increasing water deficit and rewatering. J. Plant Physiol., 157: 273-279. Go to original source...
    17. Simova-Stoilova L., Vassileva V., Petrova T., Tsenov N., Demirevska K., Feller U. (2006): Proteolytic activity in wheat leaves after drought stress and recovery. Gen. Appl. Plant Physiol., Special Issue: 91-100.
    18. Smirnoff N. (1993): The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol., 125: 27-58. Go to original source... Go to PubMed...
    19. Srivalli B., Sharma G., Khanna-Chopra R. (2003): Antioxidative defense system in an upland rice cultivar subjected to increasing intensity of water stress followed by recovery. Physiol. Plant., 119: 503-512. Go to original source...
    20. Wolff S.P. (1994): Ferrous ion oxidation in presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Methods Enzymol., 233: 182-189. Go to original source...
    21. Yadav R.S., Sharma R.K., Yadav R.M. (2004): Effect of simulated drought on free proline accumulation in some wheat (Triticum aestivum L.) genotypes. Indian J. Agric. Biochem., 17: 43-44.

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