Plant Soil Environ., 2009, 55(6):223-230 | DOI: 10.17221/1021-PSE

A comparative account of the microbial biomass-N and N-mineralization of soils under natural forest, grassland and crop field from dry tropical region, India

Jay S. Singh1, D.P. Singh1, A.K. Kashyap2
1 Department of Environmental Science, Babasaheb Bhimrao Ambedkar (Central) University, Raibareli Road, Lucknow, India
2 Department of Botany, Banaras Hindu University, Varanasi, India

This study investigated microbial biomass-N (MB-N) and N-mineralization in soils of four different vegetation systems including forest (sal), mixed forest, savanna and cropland ecosystems in the Vindhyan region, India. A change was noted in the above region due to physiographic differences and anthropogenic disturbances. Annually the soil moisture (SM) content across the different study sites ranged from 7.5 to 24.3% being maximum in forest sites compared to savanna and cropland sites. The NH4+-N, NO -3 -N and MB-N concentrations varied from 4.3 to 10.2 μg/g, 1.1 to 5.8 μg/g and 21.3 to 90.2 μg/g dry soil, respectively, with minimum values in the wet and maximum values in the dry season. The trend of seasonal variation in net N-mineralization was similar to that of moisture content but counter to the concentrations of inorganic-N and MB-N. The net N-mineralization rates at different investigated sites ranged from 4.5 to 37.6 μg/g month. Cultivation reduced the N-mineralization and MB-N by 58.5% and 63.5%, respectively. Experiments showed that the percentage contribution of MB-N to total-N was 8.01 to 19.15%. MB-N was positively correlated with the inorganic-N (n = 180,r.80,P < 0.001) but negatively with soil moisture (n = 180, r = 0.79, P < 0.001) and net N-mineralization rates (n = 180, r = 0.92, P < 0.0001). The higher N-mineralization and MB-N in the soil of forest ecosystem was reported compared to savanna and cropland and the order of soil MB-N levels and net N-mineralization followed the sequence: forest (sal) > mixed forest > savanna > cropland.

Keywords: dry tropical forest; cropland; microbial biomass; N-mineralization; soil moisture

Published: June 30, 2009  Show citation

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Singh JS, Singh DP, Kashyap AK. A comparative account of the microbial biomass-N and N-mineralization of soils under natural forest, grassland and crop field from dry tropical region, India. Plant Soil Environ. 2009;55(6):223-230. doi: 10.17221/1021-PSE.
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    References

    1. APHA (1985): Standard Methods for the Examination of Water and Wastewater. American Public Health Association, Washington D.C.
    2. Azam F.A., Farooq S., Lodhi A. (2003): Microbial biomass in agricultural soils-determination, synthesis dynamics and role in plant nutrition. Pakistan Journal of Soil Science, 6: 629-639. Go to original source...
    3. Blumfield T.J., Xu Z.H., Chen C. (2005): Mineral nitrogen dynamics following soil compaction and cultivation during hoop pine plantation establishment. Forest Ecology and Management, 204: 131-137. Go to original source...
    4. Bonde T.A., Schnurer J., Rosswall T. (1988): Microbial biomass as a fraction of potentially mineralizable nitrogen in soils from long-term field experiments. Soil Biology and Biochemistry, 20: 447-452. Go to original source...
    5. Brookes P.C., Landman A., Pruden G., Jenkinson D.S. (1985): Chloroform fumigation and the release of soil nitrogen: a rapid direct extraction method to measure microbial biomass nitrogen in soil. Soil Biology and Biochemistry, 17: 837-842. Go to original source...
    6. Buresh R.J. (1991): Extraction of ammonium, nitrate and nitrite from soil. IFD C/IRRI Collaborative Project.
    7. Chen C.R., Xu Z.H., Blumfield T.J., Hughes J.M. (2003): Soil microbial biomass during the early establishment of hoop pine plantation: seasonal variation and impacts of site preparation. Forest Ecology and Management, 186: 213-225. Go to original source...
    8. Černý J., Balík J., Kulhánek M., Nedvěd V. (2008): The changes in microbial biomass C and N in long-term field experiments. Plant, Soil and Environment, 54: 212-218. Go to original source...
    9. Eno C.F. (1960): Nitrate production in the field by incubating the soil in polyethylene bags. Soil Science Society of America Proceedings, 24: 277-279. Go to original source...
    10. Flieβbach A., Mader P. (2000): Microbial biomass and size-density fractions differ between soils of organic and conventional agricultural systems. Soil Biology and Biochemistry, 32: 757-768. Go to original source...
    11. Hassink J. (1994): Active organic matter fractions and microbial biomass as predictors of N-mineralization. European Journal of Agronomy, 3: 257-265. Go to original source...
    12. Indian Standards (1965): Indian Standards: 2720, Part IV: Grain size Analysis. Indian Standards Institute, New Delhi.
    13. Jackson M.L. (1958): Soil Chemical Analysis. Prentice Hall, New Jersey.
    14. Jaramillo V.J., Sanford J.R.R.L. (1985): Nutrient cycling in tropical deciduous forests. In: Bullock S.H., Mooney H.A., Medina E.O. (eds.): Seasonally Dry Tropical Forests. Academic Press, New York: 347-362.
    15. Lodge D.J., McDowell W.H., McSwiney C.P. (1994): The importance of nutrient pulses in tropical forest. Trends in Ecology, 9: 384-387. Go to original source... Go to PubMed...
    16. Milkha M.M., Rice C.W. (2004): Nitrogen mineralization from a soil with differing N supply capacities. Agronomy abstracts. ASA-CSSA-SSSA International Annual Meeting, 4, Seattle.
    17. Murphy P.G., Lugo A.E. (1986): Ecology of tropical dry forest. Annual Review of Ecology, Evolution and Systematics, 17: 67-88. Go to original source...
    18. Peacock A.D., Mullen M.D., Ringelberg D.B., Tyler D.D., Hedrick D.B., Gale P.M., White D.C. (2001): Soil microbial community responses to dairy manure or ammonium nitrate applications. Soil Biology and Biochemistry, 33: 1011-1019. Go to original source...
    19. Perakis S.S., Hedin L.O. (2002): Nitrogen loss from unpolluted South American forest mainly via dissolved organic compounds. Nature, 415: 416-419. Go to original source... Go to PubMed...
    20. Piper C.S. (1944): Soil and Plant Analysis. Inter Science Publication, Adelaide.
    21. Raghubanshi A.S., Srivastava S.C., Singh R.S., Singh J.S. (1990): Nutrient release in leaf litter. Nature, 346: 227. Go to original source...
    22. Raghubanshi A.S. (1991): Dynamics of soil biomass C, N and P in a dry tropical forest in India. Biology and Fertility of Soils, 12: 55-59. Go to original source...
    23. Ross D.J. (1987): Microbial biomass estimated by the fumigation-incubation procedure, seasonal fluctuations and influence of soil moisture content. Soil Biology and Biochemistry, 19: 397-404. Go to original source...
    24. Roy A., Singh K.P. (2003): Dynamics of microbial biomass and nitrogen supply during primary succession on blast furnace slag dumps in dry tropics. Soil Biology and Biochemistry, 35: 365-372. Go to original source...
    25. Sanchez P.A. (1976): Properties and Management of Soils in the Tropics. John Wiley, New York.
    26. Singh J.S., Raghubanshi A.S., Singh R.S., Srivastava S.C. (1989): Microbial biomass acts as a source of plant nutrients in dry tropical forest and savanna. Nature, 338: 499-500. Go to original source...
    27. Singh J.S., Kashyap A.K. (2006): Dynamics of viable nitrifier community, N-mineralization and nitrification in seasonally dry tropical forest and savanna. Microbiological Research, 161: 169-179. Go to original source... Go to PubMed...
    28. Singh J.S., Kashyap A.K. (2007): Contrasting pattern of methanotrophs in dry tropical forest soils: effect of soil nitrogen, carbon and moisture. Microbiological Research, 162: 276-283. Go to original source... Go to PubMed...
    29. Singh R.S., Raghubanshi A.S., Singh J.S. (1991): Nitrogen mineralization in dry tropical savanna: effects of burning and grazing. Soil Biology and Biochemistry, 23: 269-273. Go to original source...
    30. Singh S., Ghosal N., Singh K.P. (2007): Variations in soil microbial biomass and crop roots due to differing resource quality inputs in a tropical dry land agro ecosystem. Soil Biology and Biochemistr y, 39: 76-86. Go to original source...
    31. Singh S., Singh J.S. (1995): Microbial biomass associated with water stable aggregates in forest, savanna and cropland soils of a seasonally dry tropical region, India. Soil Biology and Biochemistry, 27: 1027-1033. Go to original source...
    32. Smith J.L., Paul E.A. (1990): The significance of soil microbial biomass estimations. In: Bollag J.M., Stotzky G. (eds.): Soil Biochemistry. Vol. 6. Marcel Dekker, Inc., New York, 357-396. Go to original source...
    33. Sparling G.P., Milne J.G., Vincent K.W. (1987): Effect of moisture regime on the microbial contribution to Olsen P values. Journal of Agricultural Research, 30: 79-84. Go to original source...
    34. Srivastava S.C., Singh J.S. (1989): Effect of cultivation on microbial biomass C and N of dry tropical forest soil. Biology and Fertility of Soils, 8: 343-348. Go to original source...
    35. Srivastava S.C., Singh J.S. (1991): Microbial C, N and P in dry tropical forest soils: effects of alternate land uses and nutrient flux. Soil Biology and Biochemistry, 23: 117-124. Go to original source...
    36. Statistical Graphics Corporation. (1986): Statgraphics Statistical Graphics System. USA.
    37. Tripathi N., Singh R.S. (2007): Cultivation impacts nitrogen transformation in Indian forest ecosystems. Nutrient Cycling in Agroecosystems, 77: 233-243. Go to original source...
    38. Voroney R.P., Van Veen J.A., Paul E.A. (1981): Organic C dynamics in grassland soils. II. Model validation and simulation of long-term effects of cultivation and rainfall erosion. Canadian Journal of Soil Science, 61: 211-224. Go to original source...
    39. Walkley A. (1947): A critical examination of a rapid method for determining organic carbon in soils. Effects of variations in digestion conditions and of inorganic soil constituents. Soil Science, 63: 251-264. Go to original source...
    40. Wang C., Xing X., Han X. (2004): Advances in study of factors affecting soil N mineralization in grassland ecosystems. Soil Biology and Biochemistry, 15: 2184-2188.
    41. Zhang H., Thompson M.L., Sandor J.A. (1988): Compositional differences in organic matter among cultivated and uncultivated Argiudolls and Hapludalfs derived from loess. Soil Science Society of America Journal, 52: 216-222. Go to original source...

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