Plant Soil Environ., 2009, 55(6):231-237 | DOI: 10.17221/35/2009-PSE

Chemical and microbiological characterization of Cambisols, Luvisols and Stagnosols

L. Růžek1, M. Růžková1,3, K. Voříšek1, J. Kubát2, M. Friedlová2, O. Mikanová2
1 Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic
2 Crop Research Institute, Prague, Czech Republic
3 Central Institute for Supervising and Testing in Agriculture, Brno, Czech Republic

Cambisols, Luvisols and Stagnosols are main soil taxonomical units covering 78% of the total area of the Czech Republic. In the period 2001-2008 soil samples from 13 arable and 2 grassed localities were collected and tested. Microbial biomass carbon (MBC) was determined using microwave irradiation method (MW), chloroform fumigation extraction method (FE) and re-hydration technique (RHD). Soil samples were moistened, according to the content of organic carbon (Corg), by deionized water addition (%Corg × 0.19 ml/g DM - dry mass of soil) immediately before MBC determination. Microwave sterilization (800 J/g DM = 600 W, 2 × 67 s, 100 g DM (10 soil samples) and microwave soil extracts digestion (800 J/ml = 250 W, 77 s, 24 ml) give the lowest values of MBC (204 ± 67 mg/kg DM; 100%) in comparison with FE (236 ± 57 mg/kg DM; 116%) and RHD (478 ± 138 mg/kg DM; 235%), respectively. High significant correlation (r = 0.9713) was found between TC (total carbon; 1.36 ± 0.29%) and TN (total nitrogen; 0.15 ± 0.03%) determined by CNS analyzer. Furthermore, high significant correlations were found between MBC-MW and MBC-RHD (r = 0.8965) as well as MBC-FE and DHA (dehydrogenase activity; r = 0.8094), respectively. DHA in studied soils reached 147 ± 68 mg of triphenylformazan/kg DM/24 h. Corg formed 96% of TC and total Kjeldahl nitrogen 97% of TN, respectively. According our results MW is fully acceptable for MBC determination.

Keywords: microbial biomass; total carbon and nitrogen; K2SO4 extractable carbon; dehydrogenase; Cambisols; Luvisols; Inceptisols; Alfisols

Published: June 30, 2009  Show citation

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Růžek L, Růžková M, Voříšek K, Kubát J, Friedlová M, Mikanová O. Chemical and microbiological characterization of Cambisols, Luvisols and Stagnosols. Plant Soil Environ. 2009;55(6):231-237. doi: 10.17221/35/2009-PSE.
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