Plant Soil Environ., 2018, 64(6):290-296 | DOI: 10.17221/134/2018-PSE

The first genistin absorption screening into vacuoles of Trifolium pratense L.Original Paper

Jan KUBEŠ1,2, Milan SKALICKÝ*,1, Václav HEJNÁK1, Lenka TŮMOVÁ2, Jan MARTIN2, Jaroslava MARTINKOVÁ1
1 Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
2 Faculty of Pharmacy in Hradec Králové, Charles University, Prague, Czech Republic

The determination of a transport mechanism for genistin (genistein-7-O-glucoside) across the tonoplast was performed on vacuoles from a cell culture of Trifolium pratense L. Genistin levels were examined in vacuoles as well as in an assay medium by HPLC (high-performance liquid chromatography) after treatment with various substances. MgATP increased the uptake of added genistin by 25%, but the nucleotide-free samples also contained this glycoside. Applying bafilomycin A1, an H+-ATPase inhibitor, indistinctly inhibited genistin absorption. However, vacuolar absorption of genistin was significantly reduced by N,N´-dicyclohexylcarbodiimide. This inhibitor can suppress both H+-ATPase and H+-PPase; the effect of pyrophosphate alone was not investigated. An increase in genistein levels, as result of genistin hydrolysis, could also affect the transport mechanism. The results investigated with inhibitors suggest the possible involvement of proton pumps; however additional research is required to confirm the participation of multidrug and toxin extrusion (MATE) proteins in genistin transport.

Keywords: red clover; in vitro culture; isoflavones; membrane transport; phytoestrogen

Published: June 30, 2018  Show citation

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KUBEŠ J, SKALICKÝ M, HEJNÁK V, TŮMOVÁ L, MARTIN J, MARTINKOVÁ J. The first genistin absorption screening into vacuoles of Trifolium pratense L. Plant Soil Environ. 2018;64(6):290-296. doi: 10.17221/134/2018-PSE.
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