Plant Soil Environ., 2023, 69(12):567-576 | DOI: 10.17221/394/2023-PSE

Humic acid ameliorates phytoremediation, plant growth and antioxidative enzymes in forage turnip (Brassica rapa L.)Original Paper

Sibel Boysan Canal ORCID...1, Mehmet Ali Bozkurt ORCID...1, Hilal Yílmaz ORCID...2
1 Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Van Yuzuncu Yıl University, Van, Türkiye
2 Izmit Vocational School, Department of Plant and Animal Production Kocaeli University, Kocaeli, Türkiye

In this study, the effects of ethylenediaminetetraacetic acid (EDTA) and humic acid (HA) chelate applied to soils contaminated with heavy metals on the development, antioxidant defence system, and phytoremediation of forage turnip (Brassica rapa L.) were investigated for the first. Three doses of EDTA (E1: 5 mmol/kg, E2: 10 mmol/kg, E3: 15 mmol/kg) and three doses of HA (HA1: 500 mg/kg, HA2: 1 000 mg/kg, HA3: 2 000 mg/kg) were applied to soils contaminated with heavy metals (Cd, Pb, Zn, and Cr) in the pot. According to experiment results, HA application as chelate to the polluted soil caused a significant increase in the growth of forage turnip. Phytoremediation values of the plant for Cd heavy metal were found to be BCFshoot, BCFroot > 1, and translocation factor > 1. This result proved that forage turnip has Cd accumulating properties. Also, HA application caused a decrease in H2O2 (46%) and malondialdehyde (6%) levels and antioxidative enzyme activity in polluted soil. It has been concluded that humic acid improves the oxidative stress conditions in the plant and is more effective in the development and growth of the plant than EDTA, so that it can be used effectively in phytoremediation studies.

Keywords: soil pollution; chelating substances; plant defence system; metal ions

Received: September 25, 2023; Revised: November 9, 2023; Accepted: November 13, 2023; Prepublished online: December 6, 2023; Published: December 20, 2023  Show citation

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Boysan Canal S, Bozkurt MA, Yílmaz H. Humic acid ameliorates phytoremediation, plant growth and antioxidative enzymes in forage turnip (Brassica rapa L.). Plant Soil Environ. 2023;69(12):567-576. doi: 10.17221/394/2023-PSE.
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