Plant Soil Environ., 2024, 70(11):683-694 | DOI: 10.17221/150/2024-PSE

Anti-diabetic effect of rice extract constituents through the molecular inhibition of α-amylase and α-glucosidase activityOriginal Paper

Subhashini Ramakrishnan ORCID...1, Thomas Jebastin ORCID...2, Sumathy Raj1, Ariyamuthu Ramathilaga1, Rithik Selvaraj1, Najat A. Bukhari A.3, Ashraf Atef Hatamleh3, Anis Ahamed3
1 Department of Biotechnology, Dr. G.R. Damodaran College of Science, Coimbatore, Tamil Nadu, India
2 Computer Aided Drug Designing Lab, Department of Bioinformatics, Bishop Heber College (Autonomous), Tamil Nadu, India
3 Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

Carbohydrate digestive enzymes like α-amylase and α-glucosidase can be used to treat and manage diabetes. By inhibiting these enzymes, carbohydrate digestion slowed down, lowering the level of glucose entry into the bloodstream and preventing postprandial hyperglycemia. However, the effectiveness of current antidiabetic agents is limited due to their adverse effects. Therefore, the current study explored natural inhibitors from the methanol extract of rice to combat this issue. Through an integrated approach, four different rice cultivars were analysed and found that red rice methanol extract compounds stigmasterol and 1,2-benzenedicarboxylic acid interacted with α-amylase and α-glucosidase. Additionally, further research on stigmasterol directs the structure-activity relationship studies that aid in managing diabetic conditions.

Keywords: Type2 Diabetes mellitus; hyperglycemic; carbohydrate hydrolysing enzymes; antioxidant activity; molecular interaction

Received: April 7, 2024; Revised: July 14, 2024; Accepted: July 16, 2024; Prepublished online: September 30, 2024; Published: October 17, 2024  Show citation

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Ramakrishnan S, Jebastin T, Raj S, Ramathilaga A, Selvaraj R, A. NAB, et al.. Anti-diabetic effect of rice extract constituents through the molecular inhibition of α-amylase and α-glucosidase activity. Plant Soil Environ. 2024;70(11):683-694. doi: 10.17221/150/2024-PSE.
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    References

    1. Abirami D., Gomathi R. (2022): Target and candidate agents for diabetes treatment in the framework of the food nexus. Energy Nexus, 5: 100041. Go to original source...
    2. Aneeqa B., Shagufta P., Nusrat S., Maryam R., Ahmad Mohammad S., Samir I., Mohammed B. (2024): Computational study of ADME-Tox prediction of selected phytochemicals from Punica granatum peels. Open Chemistry, 22: 1-13. Go to original source...
    3. Ashokkumar K., Govindaraj M., Vellaikumar S., Shobhana V.G., Karthikeyan A., Akilan M., Sathishkumar J. (2020): Comparative profiling of volatile compounds in popular South Indian traditional and modern rice varieties by gas chromatography-mass spectrometry analysis. Frontiers in Nutrition, 7: 599119. Go to original source... Go to PubMed...
    4. Liyanaarachchie C.P.T., Gunatilake M., Jayasinghe L., Bandra B.M.R. (2021): In vitro antidiabetic and in vivo hypoglycemic activities and toxicity of Canarium zeylanicum bark extracts. Journal of Biologically Active Products from Nature, 11: 242-253. Go to original source...
    5. Chiasson J.L., Josse R.G., Gomis R., Hanefeld M., Karasik A., Laakso M. (2002): Acarbose for prevention of type 2 diabetes mellitus: the STOP-NIDDM randomised trial. Lancet (North American Edition), 359: 2072-2077. Go to original source... Go to PubMed...
    6. Dontha S. (2016): A review on antioxidant methods. Asian Journal of Pharmaceutical and Clinical Research, 9: 14-32. Go to original source...
    7. Etsassala N.G.E.R., Badmus J.A., Marnewick J.L., Iwuoha E.I., Nchu F., Hussein A.A. (2020): Alpha-glucosidase and alpha-amylase inhibitory activities, molecular docking, and antioxidant capacities of Salvia aurita constituents. Antioxidants, 9: 1149. Go to original source... Go to PubMed...
    8. Fujisawa T., Ikegami H., Inoue K., Kawabata Y., Ogihara T. (2005): Effect of two alpha-glucosidase inhibitors, voglibose and acarbose, on post-prandial hyperglycemia correlates with subjective abdominal symptoms. Metabolism, 54: 387-390. Go to original source... Go to PubMed...
    9. Ghasemzadeh A., Karbalaii M.T., Jaafar, Asmah R. (2018): Phytochemical constituents, antioxidant activity, and antiproliferative properties of black, red, and brown rice bran. Chemistry Central Journal, 12: 17. Go to original source...
    10. Gong L., Feng D., Wang T., Ren Y., Liu Y., Wang J. (2020): Inhibitors of α-amylase and α-glucosidase: potential linkage for whole cereal foods on prevention of hyperglycemia. Food Science Nutrition, 8: 6320-6337. Go to original source...
    11. Gupta O.P., Phatak S. (2003): Pandemic trends in prevalence of diabetes mellitus and associated coronary heart disease in India - their causes and prevention. International Journal of Diabetes in Developing Countries, 23: 37-50.
    12. Harborne J.B. (2001): Twenty-five years of chemical ecology. Natural Product Reports, 18: 361-379. Go to original source... Go to PubMed...
    13. Hemamalini G., Jithesh P., Nirmala P. (2013): Phytochemical analysis of leaf extract of plant Acacia nilotica by GCMS method. Advances in Bio-logical Research, 7: 141-144.
    14. Mirmiranpour H., Ashoori M., Mikaeili A.S., Pezeshki S., Serani A., Baez A., Martirosyan D. (2022): The effect of squalene on proteinuria in patients with type 2 diabetes mellitus. Bioactive Compounds in Health and Disease, 5: 117-135. Go to original source...
    15. Ibrahim A., Umar I.A., Aimola I.A., Mohammed A. (2019): Inhibition of key enzymes linked to diabetes by Annona senegalensis Pers (An-nonaceae) leaf in vitro. Journal of Herbal Medicine, 16: 32. Go to original source...
    16. Ibrahim Z.Y., Uzairu A., Shallangwa G.A., Abechi S.E. (2021): Pharmacokinetic predictions and docking studies of substituted aryl amine-based triazolopyrimidine designed inhibitors of Plasmodium falciparum dihydroorotate dehydrogenase (PfDHODH). Future Journal of Pharmaceuti-cal Sciences, 7: 1-10. Go to original source...
    17. Jaya Prasad B., Subramania Sharavanan P., Sivaraj R. (2019): Efficiency of Oryza punctate extract on glucose regulation: inhibition of α-amylase and α-glucosidase activities. Grain and Oil Science and Technology, 2: 44-48. Go to original source...
    18. Jia Z.S., Tang M.C., Wu J.M. (1999): The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64: 555-559. Go to original source...
    19. Kashyap H., Makol V., Khatri M. (2023): Screening of phytochemicals from Derris indica for antimycobacterial potential using molecular docking analysis. Biosciences Biotechnology Research Asia, 20: 907-917. Go to original source...
    20. Krishnanunni K., Senthilvel P., Ramaiah S., Anbarasu A. (2015): Study of chemical composition and volatile compounds along with in-vitro assay of antioxidant activity of two medicinal rice varieties: Karungkuravai and Mappilai samba. Journal of Food Science and Technology, 52: 2572-2584. Go to original source... Go to PubMed...
    21. Maurus R., Begum A., Williams L.K., Fredriksen J.R., Zhang R., Withers S.G., Brayer G.D. (2008): Alternative catalytic anions differentially modu-late human alpha-amylase activity and specificity. Biochemistry, 47: 3332-3344. Go to original source... Go to PubMed...
    22. McCue P., Kwon Y.-I., Shetty K. (2005): Anti-amylase, anti-glucosidase and anti-angiotensin converting enzyme potential of selected foods. Journal of Food Biochemistry, 29: 278-294. Go to original source...
    23. Nam D.J., Kim Y., Yang E.H., Lee H.C., Ryoo J.H. (2020): Relationship between urinary phthalate metabolites and diabetes: Korean National Environmental Health Survey (KoNEHS) cycle 3 (2015-2017). Annals of Occupational and Environmental Medicine, 18: 32: e34. Go to original source... Go to PubMed...
    24. Nickavar B., Kamalinejad M., Mohandesi S. (2006): Comparison of the components of the essential oils from leaves and fruits of Grammosciadium platycarpum. Chemistry of Natural Compounds, 42: 686-688. Go to original source...
    25. Poulose N., Sajayan A., Ravindran A., Chandran A., Priyadharshini G.B., Selvin J., Kiran G.S. (2021): Anti-diabetic potential of a stigmasterol from the seaweed Gelidium spinosum and its application in the formulation of nanoemulsion conjugate for the development of functional biscuits. Frontiers in Nutrition, 8: 694362. Go to original source... Go to PubMed...
    26. Prasad M., Jayaraman S., Eladl M.A., El-Sherbiny M., Abdelrahman M.A.E., Veeraraghavan V.P., Rajagopal P.A. (2022): Comprehensive review on therapeutic perspectives of phytosterols in insulin resistance: a mechanistic approach. Molecules, 27: 1595. Go to original source... Go to PubMed...
    27. Renganathan S., Manokaran S., Vasanthakumar P., Singaravelu U., Kim P.S., Kutzner A., Heese K. (2021): Phytochemical profiling in conjunction with in vitro and in silico studies to identify human α-amylase inhibitors in Leucaena leucocephala (Lam.) De Wit for the treatment of diabetes mellitus. ACS Omega, 6: 19045-19057. Go to original source...
    28. Saeedi P., Petersohn I., Salpea P., Malanda B., Karuranga S., Unwin N., Colagiuri S., Guariguata L., Motala A.A., Ogurtsova K., Shaw J.E., Bright D., Williams R. (2019): Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045. Results from the Interna-tional Diabetes Atlas. Diabetes Research and Clinical Practice, 157: 107843. Go to original source...
    29. Sim L., Quezada-Calvillo R., Sterchi E.E., Nichols B.L., Rose D.R. (2008): Human intestinal maltase-glucoamylase: crystal structure of the N-terminal catalytic subunit and basis of inhibition and substrate specificity. Journal of Molecular Biology, 375: 782-792. Go to original source... Go to PubMed...
    30. Singleton V.L., Orthofer R., Lamuela-Raventos R.M. (1999): Analysis of total phenols and other oxidation by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299: 152-178. Go to original source...
    31. Su C.H., Hsu C.H., Ng L.T. (2013): Inhibitory potential of fatty acids on key enzymes related to type 2 diabetes. Biofactors, 39: 415-421. Go to original source... Go to PubMed...
    32. Tan D.C., Kassim N.K., Ismail I.S., Hamid M., Ahamad Bustamam M.S. (2019): Identification of antidiabetic metabolites from Paederia foetida L. twigs by gas chromatography-mass spectrometry-based metabolomics and molecular docking study. BioMed Research International, 29: 7603125. Go to original source... Go to PubMed...
    33. Widyawati T., Syarifah S., Sumantri I.B. (2021): Squalene decreased fasting blood glucose level of type ii diabetic rats. 2021. IOP Conference Series: Earth and Environmental Science, 912: 012088. Go to original source...
    34. Widyawati T., Syahputra T.A., Syarifah S., Sumantri I.B. (2023): Analysis of antidiabetic activity of squalene via in silico and in vivo assay. Molecules, 28: 3783. Go to original source...
    35. Wang J., Huang M., Yang J., Ma X., Zheng S., Deng S., Huang Y., Yang X., Zhao P. (2017): Anti-diabetic activity of stigmasterol from soybean oil by targeting the GLUT4 glucose transporter. Food Nutrition Research, 61: 1364117. Go to original source...
    36. Ward M.G., Li G., Barbosa-Lorenzi V.C., Hao M.M. (2017): Stigmasterol prevents glucolipotoxicity induced defects in glucose-stimulated insulin secretion. Scientific Reports, 7: 9536. Go to original source... Go to PubMed...
    37. Zhu X.R., Yang F.Y., Lu J., Zhang H.R., Sun R., Zhou J.B., Yang J.K. (2019): Plasma metabolomic profiling of proliferative diabetic retinopathy. Nutrition Metabolism (Lond), 16: 37. Go to original source... Go to PubMed...
    38. Wu C.T., Agrawal D.C., Huang W.Y., Hsu H.C., Yang S.J., Huang S.L., Lin Y.S. (2019): Functionality analysis of spent coffee ground extracts ob-tained by the hydrothermal method. Journal of Chemistry, 2019: 4671438. Go to original source...

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