Plant Soil Environ., 2024, 70(11):731-738 | DOI: 10.17221/318/2024-PSE

Denitrification and Anammox and Feammox in the Yinchuan Yellow River wetlandOriginal Paper

Qingsong Guan1,2,3, Yiqiao Zhou2,3, Shuo Li2,3, Fan Yang2,3, Rentao Liu1
1 Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwestern China; Key Laboratory of Restoration and Reconstruction of Degraded Ecosystems in Northwestern China of Ministry of Education, Ningxia University, Yinchuan, P.R. China
2 College of Application of Engineering, Henan University of Science and Technology, Sanmenxia, P.R. China
3 Engineering Technology Research Center of Sanmenxia Yellow River Wetland Environmental Process and Ecological Restoration, Sanmenxia, P.R. China

Denitrification, anaerobic ammonium oxidation (Anammox), and ferric iron reduction coupled with anaerobic ammonium oxidation (Feammox) are the nitrogen removal pathways in natural ecosystems. In this study, the differences between these three nitrogen removal pathways in a Phragmites australis covered site (LW), artificial grassland covered site (CD), poplar covered site (YD), and topsoil tillage after harvesting reed site (GD) in the Yinchuan Yellow River wetland were investigated using isotope tracing, metagenome, and quantitative polymerase chain reaction (Q-PCR) techniques. No 30N2 accumulation was detected in 15NH4+ addition incubations, indicating that Feammox was weak in all sites, which is consistent with a low abundance of the Feammox functional bacteria Acidimiprobiaceae sp. A6. The denitrification rates were 0.36 (LW), 0.5 (CD), 0.76 (YD) and 0.12 (GD) mg N/kg/day. The Anammox rates were 0.18 (LW) and 0.26 (GD) mg N/kg/day; other sites did not detect Anammox rate. Denitrification was the dominant pathway except for the CD site. The YD site had the highest abundance of denitrification genes, which was consistent with the denitrification rate.

Keywords: nitrogen cycling; environmental microorganism; functional gene; iron-nitrogen coupling; biogeochemistry

Received: June 11, 2024; Revised: September 5, 2024; Accepted: September 19, 2024; Prepublished online: October 9, 2024; Published: October 17, 2024  Show citation

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Guan Q, Zhou Y, Li S, Yang F, Liu R. Denitrification and Anammox and Feammox in the Yinchuan Yellow River wetland. Plant Soil Environ. 2024;70(11):731-738. doi: 10.17221/318/2024-PSE.
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