Plant Soil Environ., 2004, 50(3):95-100 | DOI: 10.17221/4013-PSE

Identification of white clover (Trifolium repens L.) cultivars using molecular markers

L. Dolanská, V. Čurn
Faculty of Agriculture, University of South Bohemia in České Budějovice, Czech Republic

The different molecular analysis for specification of white clover (Trifolium repens L.) populations was studied between 2002 and 2003. RAPD, SSR (microsatellites), rDNA and PCR-RFLP markers were used for this study. The high genetic variation was detected among the cultivars but also within the cultivars by RAPD markers. For this reason RAPD markers were not found as a suitable marker system for determination of white clover cultivars. The distribution of low genetic variation of rDNA and PCR-RFLP markers was not able to differentiate cultivars. SSR and rDNA markers did not show variability of patterns within one cultivar. The different sizes of PCR fragments were obtained after amplification with microsatellite primers. SSR markers are therefore suggested as the suitable markers for the identification of different T. repens cultivars.

Keywords: Trifolium repens L.; identification of cultivars; RAPD; microsatellites; rDNA; PCR-RFLP

Published: March 31, 2004  Show citation

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Dolanská L, Čurn V. Identification of white clover (Trifolium repens L.) cultivars using molecular markers. Plant Soil Environ. 2004;50(3):95-100. doi: 10.17221/4013-PSE.
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    References

    1. Baum B.R., Johnson D.A. (1998): The 5S rRNA gene in sea barley (Hordeum marinum Hudson sensu lato): sequence variation among repeat units and relationship to the X haplome in barley (Hordeum). Genome, 41: 652-661. Go to original source... Go to PubMed...
    2. Caetano-Anollés G., Bassam B.J., Gresshoff P.M. (1991): DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Biotechnol., 9: 553-557. Go to original source... Go to PubMed...
    3. Chen H., Sun M. (1998): Consensus multiplex PCR-restriction fragment length polymorphism (RFLP) for rapid detection of plant mitochondrial DNA polymorphism. Mol. Ecol., 7: 1553-1556. Go to original source...
    4. Dolanská L. (2002): Identifikace plané a kulturní formy jetele plazivého (Trifolium repens L.) pomocí RAPD, SSR a rDNA markerů. Coll. Sci. Pap., Fac. Agr. České Budějovice, Ser. Crop Sci., 19: 91-98.
    5. Echt C.S., Erdahl L.A., McCoy T.J. (1992): Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa. Genome, 35: 84-87. Go to original source... Go to PubMed...
    6. Gustine D.L., Huff D.R. (1999): Genetic variation within and among white clover populations from managed permanent pastures of the northeastern U.S. Crop Sci., 39: 524-530. Go to original source...
    7. Jarne P., Lagoda P.J.L. (1996): Microsatellites, from molecules to populations and back. Trends Ecol. Evol., 11: 424-429. Go to original source... Go to PubMed...
    8. Kolchinski A., Kolesnikova M., Ananiev E. (1991): "Potraying" of plant genomes using polymerase chain reaction amplification of ribosomal 5S genes. Genome, 34: 1028-1031. Go to original source... Go to PubMed...
    9. Kölliker R., Jones E.S., Drayton M.C., Dupal M.P., Forster J.W. (2001): Development and characterisation of simple sequence repeat (SSR) markers for white clover (Trifolium repens L.). Theor. Appl. Genet., 102: 416-424. Go to original source...
    10. Kollipara K.P., Singh R.J., Hymowitz T. (1997): Phylogenetic and genomic relationships in the genus Glycine Willd. based on sequences from the ITS region of nuclear rDNA. Genome, 49: 57-68. Go to original source... Go to PubMed...
    11. Marshall A.H., Michaelsonyeates T.P.T., Aluka P., Meredith M. (1995): Reproductive characters of interspecific hybrids between Trifolium repens L. and Trifolium nigrescens Viv. Heredity, 74: 136-145. Go to original source...
    12. Persson K., Diaz O., von Bothmer R. (2001): Extent and patterns of RAPD variation in landraces and cultivars of rye (Secale cereale L.) from Northern Europe. Hereditas, 134: 237-243. Go to original source... Go to PubMed...
    13. Sambrook J., Fritsch E.F., Maniatis T. (1989): Molecular cloning: a laboratory manual. Cold Spring Harbor Lab. Press, N.Y.
    14. Sweeney P.M., Dannenberger T.K. (1995): RAPD characterization of Poa annua L. populations in golf course greens and fairways. Crop Sci., 35: 1676-1680. Go to original source...
    15. Tautz D. (1989): Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucleic. Acids Res., 17: 6463-6471. Go to original source... Go to PubMed...
    16. Vejl P., Skupinová S., Sedlák P., Domkářová J. (2002): Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism. Rostl. Výr., 48: 486-489. Go to original source...
    17. Weber J.L., Wong C. (1993): Mutation of human short repeats. Hum. Mol. Gen., 2: 1123-1128. Go to original source... Go to PubMed...
    18. White T.J., Bruns T., Lee S., Taylor J. (1990): Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M., Gelfand D., Sninsky J., White T. (eds.): PCR protocols: a guide to methods and application. Acad. Press, San Diego: 315-322. Go to original source...
    19. Williams J.G.K., Kubelik A.R., Livak K.J., Rafalski J.A., Tingey S.V. (1990): DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18: 6531-6535. Go to original source... Go to PubMed...
    20. Williams W.M. (1987): White clover taxonomy and biosystematics. In: Baker M.J., Williams W.M. (eds.): White clover. CAB Int. Wallingford: 323-342.
    21. Wolfe A., Liston A. (1998): Contribution of PCR-based methods to plant systematics and evolutionary biology. In: Soltis D.E., Soltis P.S., Doyle J.J. (eds): Molecular biology of plants II DNA sequencing. Kluwer Acad. Publ., Boston, Dordrecht, London: 43-86. Go to original source...
    22. Zoldos V., Papes D., Cerbah M., Panaud O., Besendorfer V., Siljak-Yakovlev S. (1999): Molecular-cytogenetic studies of ribosomal genes and heterochromatin reveal conserved genome organization among 11 Quercus species. Theor. Appl. Genet., 99: 969-977. Go to original source...

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