Plant Soil Environ., 2006, 52(2):88-96 | DOI: 10.17221/3351-PSE

Nitrate uptake and N allocation in Triticum aestivum L. and Triticum durum Desf. seedlings

M. Trčková, Z. Stehno, I. Raimanová
Research Institute of Crop Production, Prague-Ruzyne, Czech Republic

Inter- and intra-species differences in nitrate uptake and N allocation were studied in wheat seedlings. Two collections of wheat cultivars Triticum aestivum and Triticum durum were grown at controlled conditions in hydroponics (773µM NO3-, i.e. 10.8 ppm N-NO3-). At the age of 3 weeks the net rate of nitrate uptake was measured in depletion experiments and it was expressed as µmol NO3- per g of root fresh weight per hour (µmol/g FW/h). Nitrate uptake capacity of the whole root system was expressed as µmol NO3- per plantper hour (µmol/plant/h). At the same time wheat plants were harvested and analyzed for nitrogen content. In contrast to the net rate of NO3- uptake (3.98-8.57 µmol/g FW/h) the net NO3- uptake capacity of T. aestivum roots (6.37-11.66 µmol/plant/h) significantly differed from T. durum roots (15.26-22.69 µmol/plant/h). Within T. aestivum collection cultivar Roxo exhibits the lowest value in both traits (3.98 µmol NO3-/g FW/h and 6.67 µmol NO3-/plant/h). By contrast Strela was characterized by relatively low NO3- uptake rate (5.47 µmol/g FW/h) and the highest NO3- uptake capacity (11.66 µmol/plant/h). Intra-species differences in T. durum group were not significant. In both species about 70% total nitrogen was found in shoot. Statistically significant differences in nitrogen content and its allocation were affected by growth rate in early stages of development.

Keywords: nitrate uptake rate; nitrate uptake capacity; nitrogen allocation; varietal differences; inter-species differences; wheat; Triticum aestivum; Triticum durum

Published: February 28, 2006  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Trčková M, Stehno Z, Raimanová I. Nitrate uptake and N allocation in Triticum aestivum L. and Triticum durum Desf. seedlings. Plant Soil Environ. 2006;52(2):88-96. doi: 10.17221/3351-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Crawford N.M., Glass A.D.M. (1998): Molecular and physiological aspects of nitrate uptake in plants. Trends Plant Sci., 10: 389-395. Go to original source...
    2. Filleur S., Daniel-Vedele F. (1999): Expression analysis of a high - affinity nitrate transporter isolated from Arabidopsis thaliana by differential display. Planta, 20: 461-469. Go to original source... Go to PubMed...
    3. Fraisier V., Gojon A., Tillard P., Daniel-Vedele F. (2000): Constitutive expression of a putative high-affinity nirate transporter in Nicotiana plumbaginifolia: evidence for post-transcriptional regulation by reduced nitrogen source. Plant J., 23: 489-496. Go to original source... Go to PubMed...
    4. Gebbing T., Schnyder H. (1999): Pre-anthesis reserve utilization for protein and carbohydrate synthesis in grains of wheat. Plant Physiol., 121: 871-878. Go to original source... Go to PubMed...
    5. Glass A., Siddiqi Y., Kumar A., Li W., Wang Y., Okamoto M., Unkles S., Kinghorn J.R. (2004): Nitrate uptake by plant roots: making the connections between physiology and molecular biology. In: Abstr. 7 th Int. Symp. Inorganic nitrogen assimilation in plants: from the genome to the agro-ecosystem, Wagenningen, the Netherlands.
    6. Gojon A., Dapoigny L., Lejay L., Tillard P., Rufty T.W. (1998): Effects of genetic modifications on nitrate reductase expression on 15NO3- uptake and reduction in Nicotiana plants. Plant Cell Environ., 21: 43-53. Go to original source...
    7. Jiao G., Barabas N.K., Lips S.H. (2000): Nitrate uptake, storage and reduction along the root axes of barley seedlings. In: Martins-Loucao M.A., Lips S.H. (eds.): Nitrogen in sustainable ecosystem: from the cell to the plant. Backhuys Publ., Leiden, the Netherlands: 35-38.
    8. Larsson C.M., Larsson M., Purves J.V., Clarkson C.D. (1991): Translocation and cycling through roots of recently absorbed nitrogen and suphur in wheat (Tritium aestivum) during vegetative and generative growth. Physiol. Plant., 82: 345-352. Go to original source...
    9. Oscarson P., Lundborg T., Larsson M., Larsson C.M. (1995): Genotypic differences in nitrate uptake and nitrogen utilization for spring wheat grown hydroponically. Crop Sci., 35: 1056-1062. Go to original source...
    10. Presterl T., Groh S., Landbeck M., Seitz G., Schmidt W., Geiger H.H. (2002): Nitrogen uptake and utilization efficiency of European maize hybrids developed under conditions of low and high nitrogen input. Plant Breed., 121: 480-486. Go to original source...
    11. Przulj N., Nomcilovic V. (2001): Genetic variation for dry matter and nitrogen accumulation and translocation in two-rowed barley. II. Nitrogen translocation. Eur. J. Agron., 15: 255-256. Go to original source...
    12. Quaggiotti S., Ruperti B., Borsa P., Destro T., Malagoli M. (2003): Expression of putative high-affinity NO3- transporter and of an H+-ATPase in relation to whole plant nitrate transport physiology in two maize genotypes differently responsive to low nitrogen availability. J. Exp. Bot., 384: 1023-1031. Go to original source... Go to PubMed...
    13. Stehno Z., Manev M., Dotlačil L. (1998): Grain quality characters in collection of wheat genetic resources. In: Proc. 9th Int. Wheat genetics Symp., 2: 354-356.
    14. Tischner R. (2000): Nitrate uptake and reduction in higher and lower plants. Plant Cell Environ., 23: 1005-1024. Go to original source...
    15. Tong Y., Zhou J.J., Li Z., Miller A.J. (2005): A two component high affinity nitrate uptake system in barley. Plant J., 41: 441-450. Go to original source... Go to PubMed...
    16. Trčková M., Kamínek M. (2000): Nitrate uptake and nitrogen allocation in wheat as affected by exogenous cytokinins. In: Martin-Loucao M.A., Lips S.H. (eds.): Nitrogen in sustainable ecosystem: from the cell to the plant. Backhuys Publ., Leiden, the Netherlands: 261-268.
    17. Truelman L.J., Richardson A., Forde B.G. (1996): Molecular cloning of higher plants homologues of the high affinity nitrate transporters of Chlamydomonas reinhadtii and Aspergillus nidulans. Gene, 175: 223-231. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content