Plant Soil Environ., 2009, 55(5):196-201 | DOI: 10.17221/2184-PSE

Biodegradation of propiconazole by Pseudomonas putida isolated from tea rhizosphere

S. Sarkar, S. Seenivasan, R. Premkumar
UPASI Tea Research Foundation, Tea Research Institute, Tamil Nadu, India

Biodegradation of triazole fungicide propiconazole was carried out in vitro by selected Pseudomonas strains isolated from tea rhizosphere. A total number of twelve strains were isolated and further screened based on their tolerance level to propiconazole. Four best strains were selected and further tested for their nutritional requirements. Among the different carbon sources tested glucose exhibited the highest growth promoting capacity and among nitrogen sources ammonium nitrate supported the growth to the maximum. The four selected Pseudomonas strains exhibited a range of degradation capabilities. Mineral salts medium (MSM) amended with glucose provided better environment for degradation with the highest degradation potential in strain MPR 4 followed by MPR 12 (72.8% and 67.8%, respectively).

Keywords: tea; propiconazole; Pseudomonas; biodegradation; glucose

Published: May 31, 2009  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Sarkar S, Seenivasan S, Premkumar R. Biodegradation of propiconazole by Pseudomonas putida isolated from tea rhizosphere. Plant Soil Environ. 2009;55(5):196-201. doi: 10.17221/2184-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aanes K.J., Baekken T. (1994): Acute and long-term effects of propiconazole on freshwater invertebrate communities and periphyton in experimental streams. Norwegian Journal of Agricultural Sciences Supplement, 13: 179-193.
    2. Bending G.D., Cruz M.S.R. (2007): Microbial aspects of the interaction between soil depth and biodegradation of the herbicide isoproturon. Chemosphere, 66: 664-671. Go to original source... Go to PubMed...
    3. Campbell J.I.A., Jacobsen C.S., Sørensen J. (1995): Species variation and plasmid incidence among fluorescent Pseudomonas strains isolated from agricultural and industrial soils. The Federation of European Materials Societies Microbiology Ecology, 18: 51-62. Go to original source...
    4. Douglas M.M., Dennis P.H.H. (1974): Microbial decontamination of parathion and p-nitrophenol in aqueous media. Applied Microbiology, 28: 212-217. Go to original source... Go to PubMed...
    5. Hashmi I., Khan M.A., Jong-Guk K. (2004): Malathion degradation by Pseudomonas using activated sludge treatment system (Biostimulator). Biotechnology, 3: 82-89. Go to original source...
    6. Herring I.J. (1999): The Effect of Soil Inhabiting Micro-organisms on Preservative Treated Wood. Ph.D. Thesis, Imperial College London.
    7. Hopkins G.D., McCarty P.L. (1995): Field evaluation of in situ aerobic co-metabolism of trichloroethylene and three dichloroethylene isomers using phenol and toluene as the primary substrates. Environmental Science and Technology, 29: 1628-1637. Go to original source... Go to PubMed...
    8. Hopkins G.D., Semprini L., McCarty P.L. (1993): Microcosm and in situ field studies of enhanced biotransformation of trichloroethylene by phenol-utilizing microorganisms. Applied and Environmental Microbiology, 59: 2277-2285. Go to original source... Go to PubMed...
    9. Jayaprakashvel M., Ramesh S., Mathivanan N., Baby U.I. (2006): Prevalence of fluorescent pseudomonads in the rhizosphere of plantation crops and their antagonistic properties against certain phytopathogens. Journal of Plantation Crops, 34: 728-732.
    10. Johnsen K., Andersen S., Jacobsen C.S. (1996): Phenotypic and genotypic characterization of phenanthrenedegrading fluorescent Pseudomonas biovars. Applied and Environmental Microbiology, 62: 3818-3825. Go to original source... Go to PubMed...
    11. Kaellqvist T., Romstad R. (1994): Effects of agricultural pesticides on planktonic algae and cyanobacteria: Examples of interspecies sensitivity variations. Norwegian Journal of Agricultural Sciences Supplement, 13: 117-131.
    12. Kim I.S., Beaudette L.A., Shim J.H., Trevors J.T., Suh Y.T. (2002): Environmental fate of the triazole fungicide propiconazole in a rice-paddy-soil lysimeter. Plant and Soil, 239: 321-331. Go to original source...
    13. King E.O., Ward M.K., Raney D.E. (1954): Two simple media for the demonstration of pyocyanine and fluorescein. Journal of Laboratory and Clinical Medicine, 44: 301-307. Go to PubMed...
    14. Kiyohara H., Takizawa N., Nagao K. (1992): Natural distribution of bacteria metabolizing many kinds of polycyclic aromatic hydrocarbons. Journal of Fermentation and Bioengineering, 74: 49-51. Go to original source...
    15. Mourichen X., Bengnon M. (1982): Comparative effectiveness of Tilt (CGA 64250) on banana leaf spot in the Ivory Coast. Fruits, 37: 595-597.
    16. Movahedin H., Shokoohi R., Parvaresh A., Hajia M., Jafari A.J. (2006): Evaluating the effect of glucose on phenol removal efficiency and changing the dominant microorganisms in the serial combined biological system. Journal of Research in Health Sciences, 6: 8-13.
    17. Nene Y.L., Thapliyal P.N. (1993): Fungicides in Plant Disease Control. Oxford and IBH Publishing Company (P) Limited, New Delhi.
    18. Premkumar R., Baby U.I. (1999): Tea blister blight control: Recommendations on the usage of propiconazole (Tilt 25 EC) in tea recovering from pruning. In: Handbook of Tea Culture, Section 13, UPASI Tea Science Department, Tea Research Institute, Valparai.
    19. Prescott L.M., Harley J.P., Klein D.A. (2002): Microbiology. 5 th edition. The McGraw-Hill Companies, Inc., New York.
    20. Racke K.D., Coats J.L. (1990): Enhanced biodegradation of pesticides in the environment. American Chemical Society Symposium, Series 426, Washington. Go to original source...
    21. Saaty R.P., Booth S.R. (1994): In situ bioremediation: Cost effectiveness of a remediation technology field tested at the Savannah river integrated demonstration site. LA-UR-94-1714. Los Alamos National Laboratory, Los Alamos.
    22. Skladany G.J., Metting F.B. (1993): Bioremediation of contaminated soil. In: Soil Microbial Ecology: Applications in Agricultural and Environmental Management. Marcel Dekker, New York, 483-513.
    23. Smith J.M., Speih J. (1981): Propiconazole: disease control in cereals in Western Europe. Proceedings Br. Crop Protection Conferences Insectic and Fungicides, 1: 291-297.
    24. Stolp H., Gadkari D. (1981): Non-pathogenic members of the genus Pseudomonas. In: Starr M.P., Stolp H., Truper H.G., Ballows A., Shlegel H.G. (eds): The Prokaryotes. Vol I. A handbook on habitats, isolation and identification of bacteria. Springer Verlag, Berlin, 719-741. Go to original source...
    25. Swaminathan K., Subrahmanyam P.V. (2002): Biodegradation of p-nitrophenol in anaerobic fixed film fixed bed reactor. Indian Journal of Environmental Health, 44: 8-11.
    26. Vandana P.S., Prasanth S.P. (2005): Metabolism of Carbaryl via 1, 2 Dihydroxynaphthalene by soil isolates Pseudomonas sp. strains C4, C5, and C6. Applied and Environmental Microbiology, 71: 5951-5956. Go to original source... Go to PubMed...
    27. Wallace D.F., Dickinson D. (2004): Biotransformation of Organic Biocides-longevity Versus Environmental Acceptance. COST E22 Proceeding, Brussels.
    28. Wijesinghe A.M., Knapp R.B. (1992): Preliminary feasibility and cost analysis of the in situ microbial filter concept. Lawrence, Livermore National Laboratory Report-UCRL-LD-111021.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content