Plant Soil Environ., 2010, 56(1):43-50 | DOI: 10.17221/148/2009-PSE

Effect of spring wheat (Triticum aestivum L.) treatment with brassinosteroids on the content of cadmium and lead in plant aerial biomass and grain

M. Kroutil, A. Hejtmánková, J. Lachman
Department of Chemistry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Prague, Czech Republic

Spring wheat var. Vánek was cultivated in pots in a soil naturally contaminated with heavy metals. Experimental plants were treated with three different types of brassinosteroids (BRs; 24-epibrassinolide, 24-epicastasterone and 4154) during two different growth stages 29-31 DC (off shooting) and 59-60 DC (beginning of anthesis). Content of heavy metals (Cu, Cd, Pb and Zn) was determined using AAS method in the plant growth stages 47-49 DC (visible awns), 73-75 DC (30-50% of final grain size) and 90-92 DC (full ripeness). At the stages 47-49 DC and 73-75 DC, the content of the heavy metals was determined in the biomass of whole plants, while at the stage 90-92 DC it was determined separately in straw and grains. After the treatment of plants with BRs a decrease in heavy metals content was observed in the growth stage 73-75 DC (i.e. during the period when the plants are harvested for ensilage purposes. Likewise, a decrease of lead content in the grains by 70-74% in the plants treated at both stages 29-31 DC and 59-60 DC and by 48-70% in the plants of the third group (plants treated at stage 59-60 DC) was determined as compared with the untreated plants.

Keywords: spring wheat; brassinosteroids; copper; cadmium; lead; zinc

Published: January 31, 2010  Show citation

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Kroutil M, Hejtmánková A, Lachman J. Effect of spring wheat (Triticum aestivum L.) treatment with brassinosteroids on the content of cadmium and lead in plant aerial biomass and grain. Plant Soil Environ. 2010;56(1):43-50. doi: 10.17221/148/2009-PSE.
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References

  1. Ageeva L.F., Prusakova L.D., Chizhova S.I. (2001): Effects of brassinosteroids on stalk formation and content of calcium and potassium ions in spring barley plants. Agrokhimiya, 6: 49-55.
  2. Ali B., Hasan S.A., Hayat S., Hayat Q., Yadav S., Fariduddin Q., Ahmad A. (2008): A role for brassinosteroids in the amelioration of aluminium stress through antioxidant system in mung bean (Vigna radiata L. Wilczek). Environmental and Experimental Botany, 62: 153-159. Go to original source...
  3. Ali B., Hayat S., Ahmad A. (2007): 28-Homobrassinolide ameliorates the saline stress in chickpea (Cicer arietinum L.). Environmental and Experimental Botany, 59: 33-41. Go to original source...
  4. Anuradha S., Rao S.S.R. (2007): The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress. Plant, Soil and Environment, 53: 465-472. Go to original source...
  5. Bajguz A. (2000): Blockage of heavy metals accumulation in Chlorella vulgaris cells by 24-epibrassinolide. Plant, Physiology and Biochemistry, 38: 797-801. Go to original source...
  6. Bajguz A., Tretyn A. (2003): The chemical structures and occurrence of brassinosteroids in plants. In: Hayat S., Ahmad A. (eds): Brassinosteroids. Bioactivity and Crop Productivity, Kluwer Academic Publishers, Dordrecht, 1-44. Go to original source...
  7. Cao S.Q., Xu Q.T., Cao Y.J., Qian K., An K., Zhu Y., Hu B.Z., Zhao H.F., Kuai B.K. (2005): Loss-of- function mutation in DET2 gene lead to an enhanced resistance to oxidative stress in Arabidopsis. Plant Physiology, 123: 57-66. Go to original source...
  8. Grove M.D., Spencer G.F., Rohwedder W.K., Mandava N., Worley J.F., Warthen J.D., Steffens G.L., Flippen-Anderson J.L., Cook J.C. (1979): Brassinolide, a plant growth-promoting steroid isolated from Brassica napus pollen. Nature, 281: 216-217. Go to original source...
  9. Hayat S., Ali B., Hasan S.A., Ahmad A. (2007): Brassinosteroid enhanced the level of antioxidants under cadmium stress in Brassica juncea. Environmental and Experimental Botany, 60: 33-41. Go to original source...
  10. Janeczko A., Koscielniak J., Pilipowicz M., Szarek-Lukaszewsa G., Skoczowspi A. (2005): Protection of winter rape photosystem 2 by 24-epibrassinolide under cadmium stress. Photosynthetica, 43: 293-298. Go to original source...
  11. Kagale S., Divi U.K., Krochko J.E., Keller W.A., Krishna P. (2007): Brassinosteroids confers tolerance in Arabidopsis thaliana and Brassica napus to a range of abiotic stresses. Planta, 225: 353-364. Go to original source... Go to PubMed...
  12. Khripach V.A., Voronina L.V., Malevannaya N.N. (1996): Preparation for the dimishing of heavy metals accumulation of agricultural plants. Pat. RU, 95, 101, 850.
  13. Khripach V.A., Zhabinskii V.N., de Groot A.E. (1999): Brassinosteroids: A New Class of Plant Hormones. Academic Press, San Diego, 456.
  14. Krishna P. (2003): Brassinosteroid-mediated stress responses. Journal of Plant Growth Regulation, 22: 289-297. Go to original source... Go to PubMed...
  15. Mader P., Száková J., Miholová D. (1998): Classical dry ashing of biological and agricultural materials. Part II. Loses of analytes due to their retention in an insoluble residue. Analusis, 26: 121-129. Go to original source...
  16. Nafie E.M., El-Khallal S.M. (2000): Effect of brassinolide application on growth certain metabolic activities and yield of tomato. Egyptian Journal of Physiological Sciences, 24: 103-117.
  17. Pirogovskaya G.V., Bogdevich I.M., Naumova G.V., Khripach V.A., Azizbekyan S.G., Krul L.P. (1996): New forms of mineral fertilizers with additives of plant growth regulators. Proceedings of the Plant Growth Regulation Society of America, 23: 146-151.
  18. Sakurai A., Yokota T., Clouse S.D. (1999): Brassinosteroids. Springer-Verlag, Tokyo, 253.
  19. Sharma P., Bhardwaj R. (2007a): Effects of 24-epibrassinolide on growth and metal uptake in Brassica juncea L. under copper metal stress. Acta Physiologiae Plantarum, 29: 259-263. Go to original source...
  20. Sharma P., Bhardwaj R. (2007b): Effect of 24-epibrassinolide on seed germination, seedling growth and heavy metal uptake in Brassica juncea L. General and Applied Plant Physiology 33: 59-73.
  21. Upreti K.K., Murti G.S.R. (2004): Effects of brassinosteroids on growth, nodulation, phytohormone content and nitrogenase activity in French bean under water stress. Plant Biology, 48: 407-411. Go to original source...
  22. Volynets A.P., Pshenichnaya L.A., Khripach V.A. (1997): The nature of protective action of 24-epibrassinolide on barley plants. Proceedings of the Plant Growth Regulation Society of America, 24: 133-137.
  23. Worley J.F., Mitchell J.W. (1971). Growth responses induced by brassins (fatty plant hormones) in bean plants. Journal of the American Society for Horticultural Science, 96: 270-273. Go to original source...

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