Plant Soil Environ., 2010, 56(7):312-317 | DOI: 10.17221/2/2010-PSE

Effect of salinity and radiation on proline accumulation in seeds of canola (Brassica napus L.)

M. Ashrafijou1, S.A. Sadat Noori1, A. Izadi Darbandi1, S. Saghafi2,3
1 Department of Agronomy and Plant Breeding, College of Abouraihan, University of Tehran, Tehran, Iran
2 Biophotonics Laboratory, Science and Research Branch, Plasma Physic Research Center, Islamic Azad University, Tehran, Iran
3 Bioelectronics Department, Solid State Institute, Technical University of Vienna,

Since laser beam may affect plant traits, it was used to enhance accumulation of proline in rapeseed and therefore to improve its tolerance to the salinity stress. This investigation was performed to study the effect of NaCl concentration in irrigated water (0, 100, 200 and 300 mmol NaCl) on proline accumulation of Canola (Brassica napus L.) after laser irradiation (Red, Infra-red and Nd:YAG) at two exposure treatments. In each exposure, seeds were irradiated for three minutes once or twice by the laser set. Free proline content in leaves increased significantly by increasing of NaCl concentration. Also proline content significantly increased with irradiation by laser beam. The Red laser irradiation used once and the Nd:YAG laser used twice had the greatest effect on the proline content whereas the Infrared laser had a low effect. Double application of irradiation induced a significantly higher amount of proline in the leaves compared to only one application. This is the first report on using different lasers irradiation on proline content in a winter rapeseed.

Keywords: proline-rich proteins (PRP); osmotic; salt stress; amino acids; damage plant

Published: July 31, 2010  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Ashrafijou M, Sadat Noori SA, Izadi Darbandi A, Saghafi S. Effect of salinity and radiation on proline accumulation in seeds of canola (Brassica napus L.). Plant Soil Environ. 2010;56(7):312-317. doi: 10.17221/2/2010-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abu-Elsaoud A.M., Tuleukhanov S.T., Abdel-Kader D.Z. (2008): Effect of Infra-red laser on wheat (Triticum aestivum) germination. International Journal of Agricultural Research, 3: 433-438. Go to original source...
    2. Amat A., Rigau J., Nicolau R., Aalders M., Fenoll M.R., Gemert M.V., Tomas J. (2004): Effect of Red and near-Infrared laser light on adenosine triphosphate (ATP) in the luciferine-luciferase reaction. Photochemical and Photobiology, A: Chemistry, 168: 59-65. Go to original source...
    3. Ashraf M., Foolad M.R. (2007): Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59: 206-216. Go to original source...
    4. Bates L.S., Waldren R.P., Tears I.D. (1973): Rapid determination of free proline in water stress studies. Plant and Soil, 39: 205-207. Go to original source...
    5. Bessis M., Gires F., Mayer G., Normarski G. (1962): Irradiation des organites cellulaires a I'aide d'un laser a rubis. Academy Science, Paris, 225: 1010-1012.
    6. Di Martino C., Pizzuto R. (2006): Mitochondrial transport in proline catabolism in plants: the existence of two separate translocators in mitochondria isolated from durum wheat seedling. Planta, 223: 1123-1133. Go to original source... Go to PubMed...
    7. Durkova E. (1993): The activity of wheat grains and the effect of laser radiation. Acta Phytotechnology, 49: 59-66.
    8. Girija C., Smith B.N., Swamy P.M. (2002): Interactive effects of sodium chloride and calcium chloride on the accumulation of proline and glycinebetaine in Peanut (Arachis hypogaea L.). Environmental and Experimental Botany, 47: 1-10. Go to original source...
    9. Hoffman F. (1996): Laser microbeams for the manipulation of plant cells and subcellular structures. Plant Science, 113: 1-11. Go to original source...
    10. Jofre E., Becker A. (2009): Production of succinoglycan polymer in sinorhizobium meliloti is affected by SMb21506 and requires the N-terminal domain of ExoP. Molecular Plant-Microbe Interactions, 22: 1656-1668. Go to original source... Go to PubMed...
    11. Lynikiene S., Pozeliene A. (2003): Effect of electrical field on barley seed germination stimulation. Agricultural Engineering International: the CIGR Journal of Scientific Research and Development.
    12. Matysik J., Alai Bhalu B., Mohanty P. (2002): Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Current Science, 82: 525-532.
    13. Merchan F., De Lorenzo L., Rizzo S.G. (2007): Identification of regulatory pathways involved in the reacquisition of root growth after salt stress in Medicago truncatula. Plant Journal, 51: 1-17. Go to original source... Go to PubMed...
    14. Pastore D., Greco M., Passarella S. (2000): Specific helium-neon laser sensitivity of the purified cytochrome c oxidase, International Journal of Radiate Biology, 76: 863-870. Go to original source... Go to PubMed...
    15. Pavlíková D., Pavlík M., Staszkova L., Motyka V., Száková J., Tlustoš P., Balík J. (2008): Glutamate kinase as a potential biomarker of heavy metal stress in plants. Ecotoxicology and Environmental Safety, 70: 223-230. Go to original source... Go to PubMed...
    16. Podlepeny J., Misiak L., Koper R. (2001): Concentration of free radicals in Faba Bean after the pre-sowing treatment of the seed with laser light. International Agrophysics, 15: 185-189.
    17. Qiu Z.B., Liu X., Tian X.J., Yue M. (2008): Effects of CO 2 laser pretreatment on drought stress resistance in wheat. Photochemistry and Photobiology, B: Biology, 90: 17-25. Go to original source... Go to PubMed...
    18. Roshandel P., Flowers T. (2009): The ionic effects of NaCl on physiology and gene expression in rice genotypes differing in salt tolerance. Plant and Soil, 315: 135-147. Go to original source...
    19. Rybinski W. (2000): Influence of laser beams on the variability of traits in spring barley. International Agrophysics, 14: 227-232.
    20. Rybinski W. (2001): Influence of laser beams combined with chemomutagen (MNU) on the variability of traits and mutation frequency in spring barley. International Agrophysics, 15: 115-119.
    21. Salyaev R.K., Dudareva L., Lankevich S., Makarenko S., Sumtsova V., Rudikovska E. (2007): Effect of low-intensity laser radiation on the chemical composition and structure of lipids in Wheat tissue culture. Biology Science, 412: 87-88. Go to original source... Go to PubMed...
    22. Shamseddin-Saeid M., Farahbakhsh H. (2008): Investigation of quantitative and qualitative parameters of canola under salty conditions for determining the best tolerance index. Science and Technology of Agriculture and Nature Resource, 12.
    23. Starzycki M., Rybniski W., Starzycka E., Pszczola J. (2005): Laser light as a physical factor enhancing rapeseed resistance to Blackleg disease. America's Carriers Telecommunication Association Agrophysics, 5: 441-446.
    24. Štefl M., Vašáková L. (1982): Allosteric regulation of prolineinhabitable glutamate kinase frome winter-wheat leaves by L-proline, adenosine-di-phosphate and low temperatures. Collection of Czechoslovak Chemical, 47: 360-369. Go to original source...
    25. Štefl M., Vašáková L. (1984): Regulation of proline-inhibitable glutamate kinase (EC 20702011, ATP: -L-giutamate phosphotransferase) of winter wheat leaves by monovalent cations and L-proline. Collection Czechoslovak Chemical, 49: 2698- 2708. Go to original source...
    26. Ueda A., Yamamoto-Yamane Y., Takabe T. (2007): Salt stress enhances proline utilization in the apical region of barley roots. Biochemical and Biophysical Research Communications, 355: 61-66. Go to original source... Go to PubMed...
    27. Vašáková L., Štefl M. (1982): Glutamate kinases from winter-wheat leaves and some properties of the proline-inhibitable glutamate kinase. Collection Czechoslovak Chemical, 47: 349-359. Go to original source...
    28. Vasilevski G. (1991): By laser to healthier and cheaper food. Report of Faculty of Agriculture, Skopje, 1-2.
    29. Vasilevski G. (2003): Perspectives of the application of biophysical methods in sustainable agriculture. Bulgarian Journal of Plant Physiology, Special Issue 2003, 179-186.
    30. Weber G., Stanke M., Monajembashi S., Greulich K.O. (1991): Microdissection of chromosomes of Brassica napus L. at 4000 × magnification with UV laser microbeam and stable transformation of higher plants. ISPMB Congress, Tucon, 3: 74.
    31. Zhu J.K. (2002): Salt and drought stress signal transduction in plants. Annual Review of Plant Biology, 53: 247-273. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content