Plant Soil Environ., 2010, 56(7):333-339 | DOI: 10.17221/256/2009-PSE

Soil water potential effects on the cellulase activities of soil treated with sewage sludge

A.A.S. Sinegani, A. Mahohi
Faculty of Agriculture, Bu-Ali Sina University, Hamadan, Iran

To better understand how water stress and availability affect the enzyme activity and microbial communities in soil, we measured the changes of organic carbon (OC), bacteria and fungi populations, and endoglucanase and exoglucanase activities in a semiarid soil treated with air-dried primary sewage sludge at a rate of 20 g/kg. The water potentials established for soil incubation were: saturation (SA, 0 bar), field capacity (FC, -0.3 bar), and permanent wilting point (PWP, -15 bar). An irrigation treatment was a drying-rewetting cycle (DWC) between -0.3 to -15 bars. After 0, 20, 60 and 90 days of incubation soils were sampled for analysis. The addition of sewage sludge increased soil OC, endoglucanase and exoglucanase activities significantly. The effects of soil moisture, incubation time and their interactions on OC, and endoglucanase and exoglucanase activities in soil were significant. During 20 days of incubation, OC, endoglucanase and exoglucanase activities decreased significantly. Soils incubated in DWC and FC compared to soils incubated in SA and PWP had lower OC contents due to organic matter mineralization. Organic C, exoglucanase and endoglucanase activities significantly increased with increasing soil water potential. The activities of exoglucanase and endoglucanase in soils incubated in SA were significantly higher than those in soils incubated in PWP.

Keywords: soil water potential; organic carbon; microbial population; endoglucanase and exoglucanase activity; soil incubation

Published: July 31, 2010  Show citation

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Sinegani AAS, Mahohi A. Soil water potential effects on the cellulase activities of soil treated with sewage sludge. Plant Soil Environ. 2010;56(7):333-339. doi: 10.17221/256/2009-PSE.
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    References

    1. Alef K., Nannipieri P. (1995): Methods in Applied Soil Microbiology and Biochemistry. Academic Press, London.
    2. Bayer E.A., Shimon L.J.W., Shoham Y., Lamed R. (1998): Cellulosomes structure and ultrastructure. Journal of Structural Biology, 124: 221-234. Go to original source... Go to PubMed...
    3. Burns R.G. (1982): Enzyme activity in soil: location and a possible role in microbial ecology. Soil Biology and Biochemistry, 14: 423-427. Go to original source...
    4. Duran N., Esposito E. (2000): Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil treatment: a review. Applied Catalysis B: Environmental, 28: 83-99. Go to original source...
    5. Fernandes S.A.P., Bettiol W., Cerri C.C. (2005): Effect of sewage sludge on microbial biomass, basal respiration, metabolic quotient and soil enzymatic activity. Applied Soil Ecology, 30: 65-77. Go to original source...
    6. Garcia C., Hernandez T., Costa F., Ceccanti C., Ganni A. (1993): Hydrolases in organic matter fractions of sewage sludge: changes in composting. Bioresource Technology, 44: 17-23. Go to original source...
    7. Gee G.W., Bauder J.W. (1986): Particle size analysis. In: Klute A. (ed): Method of Soil Analysis, Part 1: Physical and Mineralogical Methods. Soil Science Society America. Madison, Wisconsin, 383-411. Go to original source...
    8. Halverson L.J., Jones T.M., Firestone M.K. (2000): Release of intracellular solutes by four bacteria exposed to dilution stress. Soil Science Society of America Journal, 64: 1630-1637. Go to original source...
    9. Hesse P.R. (1971): A Text Book of Soil Chemical Analysis. John Murray, London
    10. Hinds A., Lowe L.E. (1980): Ammonium-N determination. Soil nitrogen. Berthelot reaction. Soil Science and Plant Analysis, 11: 469-475. Go to original source...
    11. Kang H., Freeman C., Lee D., Mitsch W.J. (1998): Enzyme activities in constructed wetlands: implications for water quality amelioration. Hydrobiologia, 368: 231-235. Go to original source...
    12. Killham K. (1994): Soil Ecology. Cambridge University Press, Cambridge. Go to original source...
    13. Leoppert R.H., Suarez G.L. (1996): Carbonates and gypsum. In: Sparks D.L. (ed.): Methods of Soil Analysis. Part 3: Chemical Methods. Madison, Wisconsin.
    14. Marschner P., Kandeler E., Marschner B. (2003): Structure and function of the soil microbial community in a long-term fertilizer experiment. Soil Biology and Biochemistry, 35: 453-461. Go to original source...
    15. Miller G.L. (1959): Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31: 426-428. Go to original source...
    16. Powlson D.S., Jenkinson D.S. (1976): The effects of biocidal treatments on metabolism in soil-II. Gamma irradiation, autoclaving, air-drying and fumigation. Soil Biology and Biochemistry, 8: 179-188. Go to original source...
    17. Quiquampoix H., Staunton S., Baron M.H., Ratcliffe R.G. (1993): Interpretation of the pH dependence of protein adsorption on clay mineral surfaces and its relevance to the understanding of extracellular enzyme activity in soil. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 75: 85-93. Go to original source...
    18. Ros M., Hernandez M.T., Garcia C. (2003): Soil microbial activity after restoration of a semiarid soil by organic amendments. Soil Biology and Biochemistry, 35: 463-469. Go to original source...
    19. Safari Sinegani A.A., Emtiazi G., Shariatmadari H. (2005): Sorption and immobilization of cellulose on clay minerals. Journal of Colloid and Interface Science, 290: 39-44. Go to original source... Go to PubMed...
    20. Schimel J., Balser T.C., Wallenstein M. (2007): Microbial stressresponse physiology and its implications for ecosystem function. Ecology, 88: 1386-1394. Go to original source... Go to PubMed...
    21. Skopp J., Jawson M.D., Doran J.W. (1990): Steady-state aerobic microbial activity as a function of soil water content. Soil Science Society American Journal, 54: 1619-1625. Go to original source...
    22. Sommers L.E., Nelson D.W. (1972): Determination of total phosphorus in soil: a rapid percholoric acid digestion procedure. Soil Science Society American Journal, 36: 902-904. Go to original source...
    23. Van Gestel M., Merckx R., Vlassak K. (1993): Microbial biomass responses to soil drying and rewetting: The fate of fast- and slow growing microorganisms in soils from different climates. Soil Biology and Biochemistry, 25: 109-123. Go to original source...
    24. Walkley A., Black I.A. (1934): An examination of the Degtareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Science, 37: 29-38. Go to original source...
    25. Wu J., Brookes P.C. (2005): The proportional mineralisation of microbial biomass and organic matter caused by air-drying and rewetting of a grassland soil. Soil Biology and Biochemistry, 37: 507-515. Go to original source...

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