Plant Soil Environ., 2010, 56(10):470-475 | DOI: 10.17221/54/2010-PSE

Alleviation of salt stress in citrus seedlings inoculated with mycorrhiza: changes in leaf antioxidant defense systems

Q.S. Wu1,2, Y.N. Zou1, W. Liu1, X.F. Ye1, H.F. Zai1, L.J. Zhao1
1 Collegeof Horticulture and Gardening, Yangtze University, Jingzhou City, P.R. China
2 Key Laboratory of Ecological Agriculture of Ministry of Agriculture, South China Agricultural University, Guangzhou, P.R. China

Citrus is a salt-sensitive plant. In the present study, the salt stress ameliorating the effect of arbuscular mycorrhizal fungi through antioxidant defense systems was reported. Three-month-old trifoliate orange (Poncirus trifoliata) seedlings colonized by Glomus mosseae or G. versiforme were irrigated with 0 and 100 mmol NaCl solutions. After 49 days of salinity, mycorrhizal structures were obviously restrained by salt stress. Mycorrhizal inoculation especially G. mosseae significantly alleviated the growth reduction of salinity. There were notably lower malondialdehyde and hydrogen peroxide contents in the leaves of mycorrhizal seedlings than in non-mycorrhizal ones. Mycorrhizal seedlings recorded notably greater activity of catalase and contents of ascorbate, soluble protein and glutathione under salinity or non-salinity conditions. The seedlings colonized by G. mosseae showed significantly higher antioxidant defense systems response to salinity than by G. versiforme. Our data demonstrate that mycorrhizal (especially G. mosseae) citrus seedlings exhibited greater efficient antioxidant defense systems, which provide better protection against salt damage.

Keywords: antioxidants; arbuscular mycorrhiza; reactive oxygen species; salinity; trifoliate orange

Published: October 31, 2010  Show citation

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Wu QS, Zou YN, Liu W, Ye XF, Zai HF, Zhao LJ. Alleviation of salt stress in citrus seedlings inoculated with mycorrhiza: changes in leaf antioxidant defense systems. Plant Soil Environ. 2010;56(10):470-475. doi: 10.17221/54/2010-PSE.
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    References

    1. Bradford M.M. (1976): Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-252. Go to original source...
    2. Das N., Misra M., Misra A.N. (1992): Sodium chloride salt stress induced metabolic changes in pearl millet callus: oxidases. Proceedings of the National Academy of Sciences, India, Section B, 62: 263-268.
    3. De Azevedo Neto A.D., Prisco J.T., Eneas-Filho J., Medeiros J.V.R., Gomes-Filho E. (2005): Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants. Journal of Plant Physiology, 162: 1114-1122. Go to original source... Go to PubMed...
    4. Evelin H., Kapoor R., Giri B. (2009): Arbuscular mycorrhizal fungi in alleviation of salt stress: a review. Annals of Botany, 104: 1263-1280. Go to original source... Go to PubMed...
    5. Ghorbanli M., Ebrahimzadeh H., Sharifi M. (2004): Effects of NaCl and mycorrhizal fungi on antioxidative enzymes in soybean. Biologia Plantarum, 48: 575-581. Go to original source...
    6. Giannopolitis C.N., Ries S.K. (1977): Superoxide dismutase. I. Occurrence in higher plants. Plant Physiology, 59: 309-314. Go to original source... Go to PubMed...
    7. Harinasut P., Poonsopa D., Roengmongkol K., Charoensataporn R. (2003): Salinity effects on antioxidant enzymes in mulberry cultivar. Science Asia, 29: 109-113. Go to original source...
    8. He Z.Q., He C.X., Zhang Z.B., Zou Z.R., Wang H.S. (2007): Changes of antioxidative enzymes and cell membrane osmosis in tomato colonized by arbuscular mycorrhizae under NaCl stress. Colloids and Surfaces B: Biointerfaces, 59: 128-133. Go to original source... Go to PubMed...
    9. Kar M., Mishra D. (1976): Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiology, 57: 315-319. Go to original source... Go to PubMed...
    10. Misra A.N., Latowski D., Strzalka K. (2006): The xanthophylls cycle activity in kidney bean and cabbage leaves under salinity stress. Russian Journal of Plant Physiology, 53: 102-109. Go to original source...
    11. Murkute A.A., Sharma S., Singh S.K. (2006): Studies on salt stress tolerance of citrus rootstock genotypes with arbuscular mycorrhizal fungi. Horticultural Science, 33: 70-76. Go to original source...
    12. Parniske M. (2008): Arbuscular mycorrhiza: the mother of plant root endosymbioses. Nature Reviews Microbiology, 6: 763-775. Go to original source... Go to PubMed...
    13. Phillips J.M., Hayman D.S. (1970): Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society, 55: 158-161. Go to original source...
    14. Sheng M., Tang M., Chen H., Yang B., Zhang F., Huang Y. (2008): Influence of arbuscular mycorrhizae on photosynthesis and water status of maize plants under salt stress. Mycorrhiza, 18: 287-296. Go to original source... Go to PubMed...
    15. Sudhakar C., Lakshmi A., Giridarakumar S. (2001): Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity. Plant Science, 161: 613-619. Go to original source...
    16. Tunc-Ozdemir M., Miller G., Song L., Kim J., Sodek A., Koussevitzky S., Misra A.N., Mittler R., Shintani D. (2009): Thiamin confers enhanced tolerance to oxidative stress in Arabidopsis. Plant Physiology, 151: 421-432. Go to original source... Go to PubMed...
    17. Wu Q.S., Xia R.X., Zou Y.N. (2006): Reactive oxygen metabolism in mycorrhizal and non-mycorrhizal citrus (Poncirus trifoliata) seedlings subjected to water stress. Journal of Plant Physiology, 163: 1101-1110. Go to original source... Go to PubMed...
    18. Wu Q.S., Xia R.X., Zou Y.N. (2008): Improved soil structure and citrus growth after inoculation with three arbuscular mycorrhizal fungi under drought stress. European Journal of Soil Biology, 44: 122-128. Go to original source...

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