Plant Soil Environ., 2010, 56(11):533-540 | DOI: 10.17221/190/2010-PSE

Infrared spectroscopy-based metabolomic analysis of maize growing under different nitrogen nutrition

M. Pavlík1, D. Pavlíková2, S. Vašíčková3
1 Isotope Laboratory, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic
2 Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
3 Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic

For metabolomic analysis of maize plants growing under different nitrogen nutrition sequential extraction of fresh biomass was used and isolated fractions were characterized and evaluated using IR spectra. The IR spectra of individual fractions were evaluated in relation to the nitrogen rates (2 g or 4 g N), to applied fertilizers (ammonium nitrogen or urea ammonium nitrate solution) and sampling period. For butanol fraction, typical bands of flavonoids, polar phospholipids, steryl glycosides, analogues of ecdysteroids were characterized. The IR spectra of BuOH fraction showed changes of relative contents of isolated compounds mainly affected by nitrogen rates. For water fraction bands of organic acids, salts of organic acids, flavonoid glycosides and oligopeptides (phytochelatins and/or glutathione) were the most significant. The results showed an increased induction of oxalic acid in plants after 4 g N application. Degradation of this acid induced oxidative stress, therefore strong correlation among contents of oxalic acid, flavonoids and compounds with amide nitrogen (glutathione) was observed in plants growing under 4 g nitrogen nutrition. The glutathione-ascorbate cycle protects plants against oxidative damage.

Keywords: ammonium; ecdysone phosphate; CULTAN; sequential extraction; Zea mays

Published: November 30, 2010  Show citation

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Pavlík M, Pavlíková D, Vašíčková S. Infrared spectroscopy-based metabolomic analysis of maize growing under different nitrogen nutrition. Plant Soil Environ. 2010;56(11):533-540. doi: 10.17221/190/2010-PSE.
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