Plant Soil Environ., 2011, 57(9):418-422 | DOI: 10.17221/209/2011-PSE

Nitric oxide effect on root architecture development in Malus seedlings

H.J. Gao1, H.Q. Yang2
1 Shandong Institute of Pomology, Taian, P.R. China
2 College of Horticultural Science and Engineering, Shandong Agricultural University, Taian, P.R. China

The time-dependent production of nitric oxide (NO) in roots induced by indole-3-butyric acid (IBA) and the effect of sodium nitroprusside (SNP) on root architecture development were investigated, using Malus hupehensis Rehd. seedlings. Following IBA application, a very rapid increase in NO formation and a subsequent second wave of NO burst was observed, which was related to the induction of lateral roots (LRs) and the organogenesis of lateral root primordia (LRP), respectively. The first NO burst was correlated with the second and the two peaks of NO burst induced by IBA were totally abolished by 3,3',4',5,7-pentahydroxyflavone (quercetin). Exogenous NO promoted the emergence and elongation of LR and inhibited the elongation of primary root (PR) in a dose-dependent manner: low concentrations of SNP promoted both the amounts and the elongation of LR but inhibited the elongation of LR and PR at higher concentrations. It was concluded that (i) the rapid production of NO induced by IBA was correlated with the IBA-induced initiation of LR; (ii) quercetin inhibition of IBA-induced LR formation was correlated with the quercetin inhibition of IBA-induced NO biosynthesis, and (iii) exogenous NO affects the development of root system architecture in a dose-dependent manner.

Keywords: indole-3-butyric acid; quercetin; root; sodium nitroprusside

Published: September 30, 2011  Show citation

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Gao HJ, Yang HQ. Nitric oxide effect on root architecture development in Malus seedlings. Plant Soil Environ. 2011;57(9):418-422. doi: 10.17221/209/2011-PSE.
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    References

    1. Böhm F.M.L.Z., Ferrarese M.L.L., Zanardo D.I.L., Magalhaes J.R., Ferrarese-Filho O. (2010): Nitric oxide affecting root growth, lignification and related enzymes in soybean seedlings. Acta Physiologiae Plantarum, 32: 1039-1046. Go to original source...
    2. Bradford M.M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254. Go to original source...
    3. Chhun T., Taketa S., Tsurumi S., Ichii M. (2004): Different behaviour of indole-3-acetic acid and indole-3-butyric acid in stimulating lateral root development in rice (Oryza sativa L.). Plant Growth Regulation, 43: 135-143. Go to original source...
    4. Correa-Aragunde N., Graziano M., Lamattina L. (2004): Nitric oxide plays a central role in determining lateral root development in tomato. Planta, 218: 900-905. Go to original source... Go to PubMed...
    5. Gao H.J., Yang H.Q., Wang J.Y., Wang J.X. (2010): Two enzymatic sources of nitric oxide in different organs of apple plant. Biologia Plantarum, 54: 789-792. Go to original source...
    6. Gupta K.J., Fernie A.R., Kaiser W.M., van Dongen J.T. (2011): On the origins of nitric oxide. Trends in Plant Science, 16: 160-168. Go to original source... Go to PubMed...
    7. Imin N., Nizamidin M., Wu T., Rolfe B.G. (2007): Factors involved in root formation in Medicago truncatula. Journal of Experimental Botany, 58: 439-451. Go to original source... Go to PubMed...
    8. Kolbert Z., Bartha B., Erdei L. (2008): Osmotic stress- and indole3-butyric acid-induced NO generation are partially distinct processes in root growth and development in Pisum sativum. Physiologia Plantarum, 133: 406-416. Go to original source... Go to PubMed...
    9. Lanteri M.L., Graziano M., Correa-Aragunde N., Lamattina L. (2006): From cell division to organ shape: Nitric oxide is involved in auxin-mediated root development. In: Baluška F., Mancuso S., Volkmann D. (eds.): Communication in Plants. Springer-Verlag, Berlin-Heidelberg, 123-136. Go to original source...
    10. Moreland D.E., Novitzky W.P. (1987): Interference by luteolin, quercetin, and taxifolin with chloroplast-mediated electron transport and phosphorylation. Plant and Soil, 98: 145-159. Go to original source...
    11. Nibau C., Gibbs D.J., Coates J.C. (2008): Branching out in new directions: the control of root architecture by lateral root formation. New Phytologist, 179: 595-614. Go to original source... Go to PubMed...
    12. Pagnussat G.C., Lanteri M.L., Lombardo M.C., Lamattina L. (2004): Nitric oxide mediates the indole acetic acid induction activation of a mitogen-activated protein kinase cascade involved in adventitious root development. Plant Physiology, 135: 279-286. Go to original source... Go to PubMed...
    13. Pan Q.H., Li M.J., Peng C.C., Zhang N., Zou X., Zou K.Q., Wang X.L., Yu X.C., Wang X.F., Zhang D.P. (2005): Abscisic acid activates acid invertases in developing grape berry. Physiologia Plantarum, 125: 157-170. Go to original source...
    14. Peer W.A., Murphy A.S. (2007): Flavonoids and auxin transport: modulators or regulators? Trends in Plant Science, 12: 556-563. Go to original source... Go to PubMed...
    15. Takahashi L., Sert M.A., Kelmer-Bracht A.M., Bracht A., IshiiIwamoto E.L. (1998): Effects of rutin and quercetin on mitochondrial metabolism and on ATP levels in germinating tissues of Glycine max. Plant Physiology and Biochemistry, 36: 495-501. Go to original source...
    16. Vuylsteker C., Dewaele E., Rambour S. (1998): Auxin induced lateral root formation in chicory. Annals of Botany, 81: 449-454. Go to original source...
    17. Wang P., Du Y., Li Y., Ren D., Song C.P. (2010): Hydrogen peroxidemediated activation of MAP kinase 6 modulates nitric oxide biosynthesis and signal transduction in Arabidopsis. Plant Cell, 22: 2981-2998. Go to original source... Go to PubMed...
    18. Wasson A.P., Ramsay K., Jones M.G.K., Mathesius U. (2009): Differing requirements for flavonoids during the formation of lateral roots, nodules and root knot nematode galls in Medicago truncatula. New Phytologist, 183: 167-179. Go to original source... Go to PubMed...

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