Plant Soil Environ., 2011, 57(9):403-408 | DOI: 10.17221/271/2010-PSE

Hydrogen peroxide acts as a signal molecule in CO2 laser pretreatment-induced osmotic tolerance in wheat seedling

Z.B. Qiu2, Q. Li1, Z.Z. Bi2, M. Yue1
1 College of Life Science, Northwest University, Xi'an, P.R. China
2 College of Life Science, Henan Normal University, P.R. China

The objective of this study was to test whether hydrogen peroxide (H2O2) is involved in laser pretreatment-induced water tolerance in wheat seedlings due to its nature as a second messenger in stress responses. The results showed that 3 min laser pretreatment could enhance water tolerance in wheat seedlings by decreasing the concentration of malondialdehyde (MDA), the production rate of superoxide radical (O2-), and increasing the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) and the concentration of glutathione (GSH), and having a positive physiological effect on the growth of osmotic stress seedlings. But the promotive effect of laser pretreatment-induced water tolerance in wheat seedling was effectively reversed by addition of 2 mmol AsA (ascorbic acid) or 5 µmol DPI (diphenyle iodonium), but exogenous 100 U/mL CAT could not reversed laser pretreatment-induced protective effect on wheat seedlings under osmotic stress. The results suggest that H2O2 metabolism was involved as signal in the processes of laser-induced water acclimation and laser-induced protective effect was shown to be likely related to NADPH oxidase-dependent H2O2 production.

Keywords: laser; osmotic stress; lipid peroxidation; antioxidative system

Published: September 30, 2011  Show citation

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Qiu ZB, Li Q, Bi ZZ, Yue M. Hydrogen peroxide acts as a signal molecule in CO2 laser pretreatment-induced osmotic tolerance in wheat seedling. Plant Soil Environ. 2011;57(9):403-408. doi: 10.17221/271/2010-PSE.
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